氯通道在阿霉素诱导鼻咽癌细胞凋亡中的作用  

Roles of chloride channels in apoptosis induced by adriamycin in nasopharyngeal carcinoma cells

作　　者：刘梅[1,2] 罗海[3,4] 林嘉伟[5] 魏燕[4] 李媛[2] 刘善文[2] 孟龙[2] 邹丽莉[4] 朱林燕[2] 王立伟[5,4] 陈丽新[2] 

机构地区：[1]萍乡市人民医院药学部,江西萍乡337055 [2]暨南大学医学院药理学系 [3]湖南医药学院生理学教研室,湖南怀化418000 [4]暨南大学医学院生理学系,广东广州510632 [5]暨南大学国际学院

出　　处：《中国药理学通报》2015年第9期1249-1253,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81273539;81173064;81272223);教育部博士点基金资助项目(No 20124401110009);广州市科技计划项目(No 2013J500015);东莞市科技计划项目(No 2011108102006);湖南省教育厅资助科研项目(No 12B102)

摘　　要：目的研究氯离子通道在阿霉素诱导的低分化鼻咽癌CNE-2Z细胞凋亡及凋亡性容积减小中的作用。方法采用全细胞膜片钳技术记录氯电流,流式细胞术检测细胞凋亡,活细胞图像分析法检测细胞容积变化。结果 (1)阿霉素诱导CNE-2Z细胞凋亡;(2)阿霉素诱导CNE-2Z细胞发生凋亡性容积减小,细胞外灌流阿霉素2 h,细胞容积减小约10%;(3)阿霉素可激活氯通道,产生一个有较明显外向优势的氯电流;(4)氯通道阻断剂5-nitro-2-(3-phenylpropylamino)-benzoate(NPPB)明显抑制阿霉素诱导的凋亡性容积减小、细胞凋亡和氯电流。结论阿霉素可通过激活氯通道而诱导细胞凋亡,氯通道在阿霉素诱导的鼻咽癌细胞凋亡中发挥重要作用。Aim To investigate the roles of chloride channels in the apoptosis and apoptotic volume decrease（ AVD） induced by adriamycin in nasopharyngeal carcinoma CNE-2Z cells. Methods Apoptotic rates were detected by flow cytometry,and the volume changes were measured by the time-lapse live cell imaging technique. The patch clamp technique was used to record whole-cell chloride currents. Results Adriamycin induced apoptosis of CNE-2Z cells. An early apoptotic volume decrease was observed in the cell treated with adriamycin. The cell volume was decreased by about 10% in 2 h. Adriamycin activated a chloride current which showed outward rectification. The chloride channel blocker 5-nitro-2-（ 3-phenylpropylamino）-benzoate（ NPPB） could inhibit the adriamycin-induced chloride currents,apoptosis and prevent cell shrinkage. Conclusions Our findings suggest that adriamycin causes cell apoptosis by activation of chloride channels. Chloride channels may be involved in the apoptosis and apoptotic volume decrease induced by adriamycin in CNE-2Z cells.

关 键 词：阿霉素 鼻咽肿瘤 氯通道 膜片钳技术 凋亡 凋亡性容积减小 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R739.63[医药卫生—基础医学]