PCSK9及IDOL在姜黄素促进HepG2细胞摄取LDL-C中的作用  

Role of PCSK9 and IDOL in curcumin accelerating LDL-C uptake in Hep G2 cells

作　　者：欧露[1] 张彩平[1] 刘英 乔新惠[1] 麻燕妮 欧淳 胡小波[1] 田英[1] 龙石银[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南衡阳421001 [2]常德市职业技术学院医学系,湖南常德415000

出　　处：《中国药理学通报》2015年第9期1286-1291,共6页Chinese Pharmacological Bulletin

基　　金：湖南省科技厅项目(No 2015SK2038);湖南省卫生计生委项目(No B2015-49);湖南省高层次卫生人才"225"工程项目基金;湖南省自然科学基金(No 2014JJ3104);南华大学研究生科研创新项目(No 2013XCX20);南华大学"十二五"科技创新团队基金;南华大学留学回国人员启动基金(No 2013XQD52);湖南省大学生研究性学习和创新性实验计划项目资助(No 2015-230)

摘　　要：目的从分子水平探讨姜黄素的降脂机制,为姜黄素作为降脂药物的临床开发提供科学依据。方法本研究运用油红O染色、酶法测定细胞内胆固醇含量,荧光染色检测细胞胆固醇摄取,RT-Q-PCR及Western blot检测姜黄素对HepG2细胞内胆固醇代谢相关因子在RNA和蛋白水平的表达。结果姜黄素组的HepG2细胞内红色脂滴明显增多,且TC及FC含量增高。Di I标记LDL,姜黄素组HepG2细胞橙红色荧光高于对照组。姜黄素能升高SREBP2和LDLR的mRNA和蛋白水平的表达;降低PCSK9蛋白成熟体及IDOL蛋白表达。结论姜黄素可能通过下调PCSK9及IDOL的表达,进而减少LDLR降解,促进HepG2细胞摄取LDL-C。Aim To explore the lipid-lowering mechanisms of curcumin from the molecular levels and provide scientific basis for clinical development of lipidlowering drugs. Methods Using oil red O staining and enzymic to determinate the levels of cholesterol in HepG2 cells. Moreover,uptaking of Di I-LDL was also measured. The expressions of mRNA and protein were detected by RT-Q-PCR and Western blot. Results The red lipid droplets and the levels of TC and FC significantly increased in HepG2 cells after treated with curcumin. The orange red fluorescence was higher than that of control. Curcumin could promote the expression levels of mRNA and protein of SREBP2 and LDLR,what＇s more,curcumin could reduce the expression of the mature PCSK9 level and IDOL protein. Conclusion Curcumin accelerates LDL-C uptake probably via downregulating the expression of PCSK9 and IDOL in HepG2 cells.

关 键 词：姜黄素 低密度脂蛋白受体 前蛋白转化酶枯草杆菌蛋白酶9型 诱导低密度脂蛋白受体降解蛋白 固醇调节元件结合蛋白2 3-羟基-3-甲基-戊二酰辅酶A还原酶 

分 类 号：R282.71[医药卫生—中药学] R329.24[医药卫生—中医学]