异甘草素对大鼠C6胶质瘤细胞增殖和分化的影响  

Effect of Isoliquiritigenin on C6 glioma cell proliferation and differentiation

作　　者：李雅娟[1] 甘露[2] 王占洋[1] 邱理红 佀营营 张红[2] 马成俊[3] 李忌[3] 孙喜灵[4] 王振华[3] 

机构地区：[1]石河子大学药学院,新疆石河子832005 [2]中国科学院近代物理研究所,甘肃兰州730000 [3]烟台大学生命科学学院,线粒体与健康衰老研究中心,山东烟台264005 [4]滨州医学院中西医结合学院,山东烟台264003

出　　处：《中国药理学通报》2015年第9期1298-1303,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 11175222,21372190);石河子大学重大科技攻关计划项目(No gxjs2012-zdgg02);山东省泰山学者建设工程专项经费(tshw201502046);教育部新世纪优秀人才支持计划(NCET-10-0967)

摘　　要：目的研究异甘草素对大鼠C6胶质瘤细胞增殖和分化的影响。方法采用磺酰罗丹明B(SRB)比色法和克隆形成实验考察异甘草素对C6胶质瘤细胞增殖抑制作用;Giemsa染色观察细胞形态学变化;半定量RT-PCR、Western blot鉴定细胞分化相关基因及蛋白表达水平。结果大鼠C6胶质瘤细胞经异甘草素诱导后,以浓度依赖性抑制细胞增殖(P<0.01);光镜观察到异甘草素诱导前的C6胶质瘤细胞呈两极长梭形或多角形,胞质多,胞突短;而诱导后细胞突起数目不同程度增多,细胞形态变细变长。诱导前克隆形成出现早,数量多,直径大,克隆形成率高;异甘草素处理组克隆形成率降低,与对照组相比差异有统计学意义(P<0.01)。RT-PCR、Western blot结果显示,异甘草素诱导后C6细胞GFAP mRNA和蛋白表达均明显上升(P<0.01)。结论异甘草素能抑制大鼠C6胶质瘤细胞的增殖,并能诱导C6细胞分化。Aim To investigate the effects of isoliquiritigenin（ ISL） on C6 glioma cell proliferation and differentiation. Methods C6 glioma cells ＇ viability and proliferation were respectively measured by SRB test.Colony formation of C6 glioma cells from different groups was assayed. After culturing the cells from each group,giemsa staining was used to observe cell morphology. RT-PCR was applied to detect mRNA expression of GFAP. Western blot was applied to detect the expression of GFAP. Results ISL effectively inhibited the viability of C6 glioma cells when compared with the control group in a concentration-dependent manner（ P〈0. 01）. The morphological observation under light microscope showed that： in the control group,most of the undifferentiated C6 cells showed long fusiform and polygonal shape. Compared to the control group,the C6 cells treated with ISL revealed alteration in morphology such as astrocytes with smaller smooth,round body and much finer longer,tapering processes. The cloning formation rate detection revealed that： the colonies in the control group semerged earlier and were larger than those experimental ones,the cloning formation rate was higher,while almost no effective cells colony emerged in ISL treated groups（ P〈0. 01）. Western blot and RTPCR analysis showed that GFAP expression in the experimental groups increased（ P〈0. 01）. Conclusion ISL may inhibit the proliferation of C6 glioma cells and induce their differentiation.

关 键 词：异甘草素 C6胶质瘤 增殖 分化 克隆形成 吉姆萨染色 星形胶质细胞 GFAP 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]