蒺藜皂苷对局灶性脑缺血大鼠炎症反应和血脑屏障通透性的影响  

Effects of Gross Saponins of Tribulus terrestris L. on Inflammatory Reaction and Permeability of Blood-brain Barrier in Rats Following Cerebral Ischemic Injury

作　　者：翟凤国[1] 周福波[1] 李厚忠[1] 郭素芬[1] 林峰[1] 关利新[1] 

机构地区：[1]牡丹江医学院药理学教研室,牡丹江157011

出　　处：《医药导报》2015年第9期1131-1134,共4页Herald of Medicine

基　　金：黑龙江省教育厅科学技术研究项目(11551527);黑龙江省卫生厅科研课题(2012-276);牡丹江医学院科学技术研究项目(2010-02)

摘　　要：目的探讨蒺藜皂苷对大鼠脑缺血-再灌注损伤炎症反应和血脑屏障通透性的作用及其机制。方法斯泼累格·多雷(SD)大鼠60只,随机分为假手术组、模型对照组、蒺藜皂苷小剂量组(10 mg·kg-1)、蒺藜皂苷大剂量组(30 mg·kg-1),每组15只,采用线栓法制备脑缺血-再灌注损伤模型。缺血2 h再灌注24 h后分别检测大鼠神经功能损伤评分、缺血脑组织髓过氧化物酶(MPO)的活性和伊文思蓝(EB)的含量;采用酶联免疫吸附测定(ELISA)法检测脑组织肿瘤坏死因子-α(TNF-α)的含量,免疫印记法检测基质金属蛋白酶-9(MMP-9)的表达变化。结果与模型对照组比较,蒺藜皂苷小、大剂量组大鼠神经功能损伤减轻(P<0.05),缺血脑组织MPO活性和EB含量均明显降低(P<0.05或P<0.01),TNF-α含量明显降低[分别为(0.760±0.110),(0.670±0.073)mg·g-1,模型对照组为(0.920±0.128)mg·g-1,P<0.05或P<0.01)],MMP-9的表达水平均明显降低[分别为(1.770±0.181)%,(1.480±0.146)%,模型对照组为(2.200±0.186)%,P<0.01]。结论蒺藜皂苷对大鼠脑缺血-再灌注损伤的脑组织具有神经保护作用,其机制可能与降低TNF-α含量和下调MMP-9表达,从而降低炎症反应和血脑屏障通透性有关。Objective To explore the effects of gross saponins of Tribulus terrestris L. on inflammatory reaction and permeability of blood-brain barrier in rats following cerebral ischemia-reperfusion injury and their potential mechanisms.Methods Sixty SD rats were divided into sham operation group,model control group,gross saponins of Tribulus terrestris L. at low-dose( 10 mg·kg-1) and high-dose groups( 30 mg·kg-1).Cerebral ischemia-reperfusion model was established with suture emboli method in middle cerebral artery of rats. Neural injury scores,the contents of Evans blue( EB) and myeloperoxidase(MPO) activities in rat brain were measured 24 hours after the cerebral reperfusion post 2 h ischemia. Content of tumor necrosis factor-α( TNF-α) in rat brain was detected by ELISA; expression levels of matrix metalloproteinase-9( MMP-9) in rat brain was determined by Western blot. Results Compared to the model control group,the neurological deficit scores were significantly decreased( P<0.05),MPO activities and EB contents decreased( P < 0. 05 or P < 0. 01) in the treatment groups. The expression levels of TNF-α were significantly lower in the treatment groups( 0.760±0.110) mg·g-1and( 0.670±0.073) mg·g-1compared to( 0.920±0.128) mg · g-1in the model control group( P < 0. 05 or P < 0. 01). The MMP-9 expression levels were( 1.770±0.181) % and( 1.480±0.146) %,significantly lower than( 2.200±0.186) % in the model control group( P<0.01). Conclusion Gross saponins of Tribulus terrestris L. exert neuroprotective effects on cerebral ischemia-reperfusion injury in rats through inhibiting the inflammatory reaction and decreasing the permeability of blood-brain barrier,which may be associated with the decrease of the TNF-α content and downregulation of the MMP-9 expression.

关 键 词：蒺藜皂苷 缺血-再灌注 脑 炎症 血脑屏障 基质金属蛋白酶 

分 类 号：R282.71[医药卫生—中药学] R285[医药卫生—中医学]