葛根素对PI3K/AKT通路介导PASMCS凋亡的影响  

Effect of puerarin on PI3K / AKT pathway-mediated apoptosis of PASMCs

作　　者：张晓丹[1] 杨艳南[1] 王淑静[1] 朱大岭[2] 王力维[1] 盛洁静[1] 宋莎莎[2] 

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076 [2]哈尔滨医科大学大庆校区,黑龙江大庆163319

出　　处：《中国中药杂志》2015年第15期3041-3046,共6页China Journal of Chinese Materia Medica

基　　金：哈尔滨市科技创新人才项目(2013RFXXJ092);黑龙江省自然科学基金重点项目(2D201416)

摘　　要：探讨葛根素(puerarin,Pue)对缺氧抑制的大鼠肺动脉平滑肌细胞(PASMCs)凋亡的影响,并研究细胞外信号PI3K/AKT通路是否参与了Pue诱导PASMCs凋亡的过程。以血清饥饿组(SD组)为对照组,通过噻唑蓝比色法(MTT)、Annexin VFITC凋亡检测试剂盒和蛋白免疫印迹法检测Pue对大鼠PASMCs凋亡情况的影响。采用蛋白免疫印记法检测PI3K/AKT通路是否参与了Pue诱导缺氧抑制的PASMCs凋亡的进程中。结果显示,1常氧条件下Pue对PASMCs细胞凋亡无影响,缺氧可抑制PASMCs凋亡,常氧及缺氧条件下Pue对TNF-α表达无影响。Pue可以逆转缺氧诱导的Bcl-2(P<0.01)表达上调以及Bax(P<0.01)表达下调。2常氧情况下Pue对P-AKT的表达没有影响,LY294002与Pue均可抑制缺氧诱导的Bcl-2,P-AKT表达上调以及Bax表达下调,与缺氧单独加Pue或缺氧单独加入LY294002对比,缺氧+Pue+LY294002组Bcl-2,Bax,P-AKT表达变化更显著。结果表明,Pue可诱导缺氧抑制的PASMCs凋亡,并且可能是通过PI3K/AKT通路调控的。To discuss the effect of puerarin（ Pue） on the proliferation of hypoxia-induced pulmonary artery smooth muscle cells（ PASMCs） and discuss whether the extracellular signal PI3K/AKT pathway was involved in the Pue-induced PASMC apoptosis. With the serum starvation group（ SD group） as the control group,the MTT colorimetry method,Annexin V-FITC apoptosis detection kit and Western blot were used to detect Pue＇s effect on apoptosis of rat PASMCs. The protein immunoblot assay was used to detect whether PI3 K / AKT pathway was involved in the inhibition of hypoxia-induced PASMC apoptosis process. The results show that under normoxic conditions,Pue had no effect on PASMC apoptosis; Under hypoxia conditions,Pue can inhibit PASMC apoptosis; Under normoxic and hypoxic conditions,Pue had no effect on TNF-α expression. Pue can reverse hypoxia-induced Bcl-2（ P〈0. 01）,up-regulate it and down-regulated Bax（ P〈0. 01）. Under normoxic conditions,Pue had no effect on P-AKT expression. Both LY294002 and Pue can inhibit hypoxia-induced Bcl-2, up-regulation of P-AKT expression and down-regulation of Bax expression. Compared with the hypoxia ＋ Pue group or the hypoxia ＋ LY294002 group,the hypoxia ＋ Pue ＋ LY294002 group showed more significantly changes in Bcl-2,Bax,P-AKT expressions. The results show that,Pue can inhibit the hypoxic-induced PASMC apoptosis,which may be regulated through PI3 K / AKT pathway.

关 键 词：葛根素 PASMCs BCL-2 BAX P-AKT 

分 类 号：R285[医药卫生—中药学]