壮腰健肾丸对肾虚多尿大鼠膀胱组织中P2X_1与P2X_3 mRNA表达的影响  

Effect of Zhuangyao Jianshen Wan( ZYJCW) on P2X_1and P2X_3 mRNA expressions in rats with diuresis caused by kidney deficiency

作　　者：陈家艺[1] 江伟雯 何风雷 莫国强 郭钟慧 王笑丹 吴清和[2] 操红缨[2] 

机构地区：[1]湖北民族学院附属民大医院,湖北恩施445000 [2]广州中医药大学,广东广州510405 [3]广州白云山陈李济药厂有限公司,广东广州510290

出　　处：《中国中药杂志》2015年第15期3059-3062,共4页China Journal of Chinese Materia Medica

基　　金：中央财政支持地方高校发展专项资金项目(财教[2013]338号)

摘　　要：为了探讨壮腰健肾丸的缩尿作用及机制,该实验采用皮下注射150 mg·kg-1剂量的D-半乳糖诱导亚急性衰老模型,并将其分为模型组、缩泉丸组(1.17 g·kg-1·d-1)、壮腰健肾丸高、中、低剂量组(2.39,1.20,0.60 g·kg-1·d-1),另取正常动物设为空白组,连续给药8周。选用代谢笼法测量大鼠24 h尿量及水负荷5 h尿量;采用全自动生化仪检测尿中Na+,Cl-,K+浓度;应用ELISA法测定血清中醛固酮(aldosterone,ALD)、抗利尿激素(antidiuretic hormone,ADH)的含量;采用RT-PCR检测大鼠膀胱组织中P2X1与P2X3mRNA表达量的变化情况。结果显示壮腰健肾丸高、中剂量组对肾虚多尿大鼠24 h尿量及水负荷后5 h内尿量均显著下调(P<0.05,P<0.01);对尿中Na+和Cl-浓度均显著降低(P<0.01);血浆中ALD,ADH含量显著性升高(P<0.05,P<0.01);膀胱组织中P2X1与P2X3mRNA表达量显著下调(P<0.01);壮腰健肾丸低剂量组尿中Na+和Cl-浓度均显著降低(P<0.01)。结果说明壮腰健肾丸可能是通过下调肾虚多尿大鼠膀胱组织中P2X1与P2X3mRNA表达量从而发挥缩尿的作用。To investigate the urination-reducing effect and mechanism of Zhuangyao Jianshen Wan（ ZYJCW）. In this study,SD rats were subcutaneously injected with 150 mg·kg-1dose of D-galactose to prepare the sub-acute aging model and randomly divided into the model group,the Suoquan Wan group（ 1. 17 g·kg-1·d-1）,and ZYJCW high,medium and low dose groups（ 2. 39,1. 20,0. 60 g·kg-1·d-1）,with normal rats in the blank group. They were continuously administered with drugs for eight weeks. The metabolic cage method was adopted to measure the 24 h urine volume and 5 h water load urine volume in rats. The automatic biochemistry analyzer was adopted to detect urine concentrations of Na＋,Cl-,K＋. The ELISA method was used to determine serum aldosterone（ ALD） and antidiuretic hormone（ ADH）. The changes in P2X1 and P2X3mRNA expressions in bladder tissues of rats were detected by RT-PCR. According to the results,both ZYJCW high and medium dose groups showed significant down-regulations in 24 h urine volume and 5 h water load urine volume in（ P〈0. 05,P〈0. 01）,declines in Na＋and Cl-concentrations in urine（ P〈0. 01）,notable rises in plasma ALD and ADH contents（ P〈0. 05,P〈0. 01） and remarkable down-regulations in the P2X1 and P2X3mRNA expressions in bladder tissues（ P〈0. 01）. The ZYJCW low dose group revealed obvious reductions in Na＋and Cl-concentrations in urine（ P〈0. 01）. The results indicated that ZYJCW may show the urination-reducing effect by down-regulating the P2X1 and P2X3 mRNA expressions in bladder tissues of rats with diuresis caused by kidney deficiency.

关 键 词：壮腰健肾丸 肾虚多尿 P2X1 P2X3 

分 类 号：R285.5[医药卫生—中药学]