五倍子内生菌的分离与鉴定  

Isolation and identification of endophytes in Galla chinensis

作　　者：林丽美[1] 张华玲[1] 李顺祥[1] 肖嫩群[1] 谭周进[1] 曾晓艳[1] 梁晓 龚力民[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]长沙市中医医院,湖南长沙410000

出　　处：《中国微生态学杂志》2015年第8期874-877,共4页Chinese Journal of Microecology

基　　金：长沙市科技局课题(K1301005-31);教育部高等学校博士学科点专项科研基金(20124323120004);湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金资助项目(ZYFT201310);湖南省大学生研究性学习和创新性实验计划项目(2013173);湖南省十二五药学重点学科(2011年)

摘　　要：目的分离五倍子内生菌,并进行筛选和鉴定。方法分别采用牛肉膏蛋白胨固体、液体培养基,高氏1号培养基,马丁孟加拉红培养基外加4‰的国产硫酸庆大霉素(800万单位)四种培养基对五倍子内生菌进行分离。结果得到5株菌株,经菌落形态显微观察与16S rDNA序列分析鉴定,5株菌株均为革兰阳性菌;A1、A5和B1为杆菌,同属芽胞杆菌属;A3为球菌,微球菌属;A6为球菌,葡萄球菌属;A5与B1同源性最高,达到100%。结论从五倍子中分离得到5株内生细菌,将有利于五倍子的深入开发利用。Objective To isolate,screen and identify endophytes fromGalla chinensis.Methods Four different isolation media were used to isolate the endophytes.Results Five strains of endophytes were obtained.Morphological observation and 16 S rDNA sequence analysis showed that they were all gram positive microbes.The strains A1,A5 and B1were identified as Bacillus subtilis,A3 was Micrococcus luteus,while A6 was Staphylococcus sciuri.The highest homology was 100% between A5 and B1.Conclusion Five endophytes were isolated fromGalla chinensis,which will be beneficial to the further development and utilization of Galla chinensis.

关 键 词：五倍子 内生菌 16S RDNA 分离 鉴定 

分 类 号：R378[医药卫生—病原生物学]