通莲汤对裸小鼠胃癌移植瘤生长及缺氧诱导因子-1α的影响  被引量:2

Tonglian Decoction on tumor growth and HIF-1α expression of tumor tissue from nude mice modeled with gastric cancer cells

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作  者:张艳丽 贾永森[2] 秦丽娟[3] 孙瑞军[3] 

机构地区:[1]迁安燕山医院,河北迁安064400 [2]河北联合大学中医学院,河北唐山063000 [3]河北联合大学基础医学院,河北唐山063000

出  处:《吉林中医药》2015年第8期837-840,共4页Jilin Journal of Chinese Medicine

基  金:河北省自然科学基金(H2013209053)

摘  要:目的探讨复方通莲汤对胃癌MGC803细胞荷瘤裸鼠的抑瘤作用及对缺氧诱导因子-1α(HIF-1α)表达的影响,为该方临床应用提供实验依据。方法胃癌细胞株MGC803接种于裸鼠右前肢皮下。模型组灌胃生理盐水溶液10 m L/kg,阳性对照组灌胃消癌平片1.8 g(生药量)/kg的药液10 m L/kg,复方通莲汤高、中、低剂量组分别灌胃1.5,0.75,0.375 g(生药量)/L的药液10 m L/kg,各组连续给药3周,再连续观察3周,6周后处死小鼠,分离瘤组织,计算抑瘤率,免疫组织化学法检测肿瘤组织HIF-1α表达。结果高剂量通莲汤显著抑瘤生长,瘤重(1.51±0.49)g,抑瘤率为(61.04±4.79)%,与模型组比较差异有统计学意义(P<0.05);通莲汤可显著抑制HIF-1α表达,与模型组比较,阳性表达率χ2=4.286,P=0.038<0.05。结论通莲汤可抑制裸鼠人胃癌MGC803细胞移植瘤生长,其抑瘤机制与下调肿瘤细胞HIF-1α蛋白表达有关。Objective The purpose of the research is to observe inhibition of Tonglian Decoction( TD) on nude mice modeled with gastric cancer cells MGC-803 and on hypoxia inducible factor-1α( HIF-1α) expression in tumor tissue,to provide experimental basis for clinical application of TD. Methods MGC-803 cells are inoculated subcutaneously in right forelimbs of all mice. Mice in model group are treated with saline solution 10 m L / kg orally. Mice in positive control group are treated with Xiaoaiping tablet,dosage of 10 m L / kg,1. 8 g / kg( crude drug) of liquid. Mice in TD groups are subdivided as high( 1.5 g / L),medium( 0. 75 g / L) and low( 0. 375 g / L) dose group of drug liquid,with dosage of 10 m L / kg. All mice take administration for 3 weeks and are observed for another 3 weeks continuously. Tumor volumes are measured for 6 weeks everyday from the beginning of MGC803 cells transplanted. All mice are killed for isolation of tumor tissue,inhibition ratio of tumor-weight measured. Finally,HIF-1α expression is examined with immunohistochemistry. Results Tumor weight in TD high-dose group is( 1. 51 ± 0. 49) g,and the inhibition ratio is( 61. 04 ± 4. 79) %,which is significantly different from that in the model group( P〈 0. 05); Positive rate of HIF-1α expression also decreased significantly,showing statistical difference from the model group( χ^2= 4. 286,P = 0. 038〈 0. 05). Conclusion TD inhibits tumor growth on nude mice modeled with MGC803,through downregulation of HIF-1α protein expression.

关 键 词:通莲汤 胃癌 MGC803细胞 荷瘤裸鼠 缺氧诱导因子-1Α 

分 类 号:R285.5[医药卫生—中药学]

 

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