针刺对血管性痴呆大鼠Bax和Bcl-2表达的影响  

Effect of Needling on Bax/Bcl-2 signaling pathway of rats with vascular dementia

作　　者：鹿俊磊[1] 顿玲露 卢昌均[1] 曾鉴源[1] 吴玉芬[1] 王少洲[1] 徐榛[1] 赵世亮[1] 

机构地区：[1]柳州市中医院,柳州545001

出　　处：《吉林中医药》2015年第8期841-843,共3页Jilin Journal of Chinese Medicine

基　　金：国家自然基金地区科学基金(81160472);广西自然科学基金(2012GXNSFAA053165);广西中医药大学自然科学研究课题(P2012109;P2012107);柳州市科技创新能力与条件建设项目(2013F010401)

摘　　要：目的探讨针刺对血管性痴呆大鼠血清中Bax和Bcl-2表达的影响。方法将大鼠随机分为假手术组、模型组和针刺组。间断夹闭颈总动脉法建立Va D模型,Morris水迷宫实验检测模型是否成功,酶联免疫吸附法分析(ELISA)检测血清中Bax和Bcl-2的含量。结果针刺治疗后Bax表达较模型组〔(410.176±65.942)pg/m L比(612.353±92.554)pg/m L〕下降(P<0.01),Bcl-2较模型组表达〔(275.509±60.833)pg/m L比(119.246±20.562)pg/m L〕明显升高(P<0.01)。结论针刺可通过上调大鼠Bcl-2水平,下调Bax表达,对抗脑缺血所致的细胞凋亡,防止继发性损伤,达到促进神经修复的作用。Objective To investigate the role of needling affect to expression of Bax and Bcl-2 of the rats after vascular dementia（ Va D）. Methods The rats were randomly divided into sham operation group,Va D model group and Needling treatment group. The rat model of Va D was built by intermittently clipping common carotid artery. Morris water maze test whether the surgery had been successful and detection the changes of Bax and Bcl-2 by Enzyme-linked immunosorbent assay（ ELISA）. Results Bax expression（ 410. 176 ± 65. 942） pg / m L compare（ 612. 353 ± 92. 554）pg / m L decreased after acupuncture treatment（ P〈 0. 01）,the Bcl-2 expression（ 275. 509 ± 60. 833） pg / m L compares（ 119. 246 ± 20. 562） pg / m L increased significantly,Bax / Bcl-2 ratio decreased compared with model group（ P 〈0. 01）. Conclusion The acupuncture therapy on vascular dementia maybe related to up-regulated the expression of Bcl-2 protein in rat and decreased Bax protein expression thus regulate anti-apoptotic effect and promotes neurological function recovery in cerebral ischemia injury and prevent secondary injury,to promote the role of nerve repair.

关 键 词：针刺 血管性痴呆 BAX蛋白 BCL-2蛋白 大鼠 

分 类 号：R259[医药卫生—中西医结合]