广西巴马小型猪转基因克隆胚胎的体外生产  

In vitro Production of Guangxi Bama Mini-pig Transgenic Cloned Embryos

作　　者：朱向星[1,2] 全守能 卢晟盛[1,2] 

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室,南宁530004 [2]广西大学动物科学技术学院,南宁530004

出　　处：《基因组学与应用生物学》2015年第1期41-46,共6页Genomics and Applied Biology

基　　金：国家自然科学基金资助项目(31260553);广西硕士研究生创新课题(YCSZ2013003)共同资助

摘　　要：广西巴马小型猪是一种原产于广西巴马县的小型猪品种,非常适于实验动物化,进行广西巴马小型猪的基因修饰研究,可以显著提升其在生物医学研究中的利用价值。当前最有效的构建转基因猪方法是体细胞核移植,但因用于体细胞核移植生物供体细胞在经受转基因操作后活性显著下降,从而制约了转基因克隆猪的生产效率。Xfect polymer是一种新型转染试剂,具有细胞毒性低和操作简便等优点,已被证明适用于多种真核细胞的转基因操作。本研究旨在利用体细胞核移植技术来检验经Xfect polymer转染制备的广西巴马小型猪转基因体细胞,可否支持猪克隆胚胎完全的体外发育的能力,以期为将来生产模拟人类疾病的转基因克隆广西巴马小型猪奠定基础。Guangxi Bama mini-pig,a distinct mini-pig species originating in Guangxi Bama County,is very suitable for experimental animalized.The application value of Guangxi Bama mini-pig in biomedical research could be significantly enhanced by genetic modification.Somatic cell nuclear transfer（SCNT）,the presently most widely used strategy to generate transgenic pigs,is unfortunately limited by the decline of cellular activity of nuclear transfer donor cells suffered genetically modified operation.Xfect polymer,a novel transfection reagent enjoys the advantages of minimal cytotoxicity and user-friendly protocols,has been demonstrated be suitable for genetic modification of a large number types of eukaryocytes.This present study was conducted,via SCNT,to examine whether these Guangxi Bama mini-pig transgenic fibroblasts established by using the Xfect polymer transfection be capable of supporting complete development of porcine cloned embryos in vitro,therefore lay the foundation for producing transgenic cloned Guangxi Bama mini-pigs as human disease models.

关 键 词：广西巴马小型猪 胚胎 体细胞核移植 基因修饰 转染 

分 类 号：Q813[生物学—生物工程]