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作 者:赵新春[1,2] 贾智英[1] 李池陶[1] 石连玉[1]
机构地区:[1]中国水产科学研究院黑龙江水产研究所,哈尔滨150070 [2]上海海洋大学水产与生命学院,上海201306
出 处:《基因组学与应用生物学》2015年第1期90-95,共6页Genomics and Applied Biology
基 金:现代农业产业技术体系建设专项资金(CARS-46-02);国家科技支撑计划资助(2012BAD26B02)共同资助
摘 要:本研究从鲤优质性状选育群体F3代中随机选取96尾个体,并且对所选取的所有个体进行PIT标记。利用12对具有较高多态性的微卫星标记对所选取个体的遗传结构进行分析。结果表明:群体平均等位基因数(A)为6.166 7,有效等位基因数(Ae)为4.158 6,期望杂合度(He)值为0.741 8,平均多态信息含量(PIC)为0.702 3,显示出该选育群体具有丰富的遗传多样性;通过对12个位点进行哈迪-温伯格平衡检验结果显示其中有8个位点显著或极显著偏离平衡,这可能是人工选择压力的结果;有效群体大小和瓶颈效应分析表明,有效群体大小为82.9,该群体在近期内可能经历了遗传瓶颈。本研究通过对选育群体F3的遗传变异进行分析,初步了解了该种群的遗传结构,为选育群体F3的进一步繁育提供理论支持。In this study we randomly selected 96 individual F3 carps in the quality breeding population,and all selected individuals were marked with PIT.The genetic structure of a new breeding variety of quality carp was studied by using 12 polymorphic microsatellite loci.The results showed that the colonial average number of alleles was 6.166 7 and the effective of alleles was 4.158 6.The average of expected heterozygosity(He) was 0.741 8,and the average of polymorphic information content(PIC) was 0.702 3.The results indicated rich polymorphism information content and large genetic diversity in the population.The analysis of Hardy-Weinberg equilibrium indicated that 8 loci had deviated from equilibrium,and the cause of this kind of deviation phenomenon might come from artificial selection.The estimation of effect population size(Ne) and bottleneck gave the results that Ne was82.9 and bottleneck effect had occurred recently.The aim of the study was to discover the genetic structure of the new variety,which would be important for the protection and utilization of the germplasm.In this study,by analyzing the genetic variation of quality F3 carp breeding populations a preliminary understanding of the genetic structure of the population,and provide theoretical support for further breeding quality carp through.
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