乳糖诱导剂及培养条件对C型产气荚膜梭菌茁β_2毒素基因表达影响  

The Influences of Lactose Inducer and Culture Conditions on the Expression of the beta2-toxin Gene of Clostridium perfringens Type C

作　　者：许崇利[1] 

机构地区：[1]吉林化工学院生物与食品工程学院,吉林132022

出　　处：《基因组学与应用生物学》2015年第1期112-116,共5页Genomics and Applied Biology

基　　金：国家自然科学基金项目(30972188);吉林化工学院博士启动基金项目共同资助

摘　　要：本研究对已构建的含茁2毒素基因重组质粒pE TXB2进行限制性核酸内切酶酶切鉴定,并用SDS-PAGE检测在不同培养条件下茁2毒素基因的表达情况。经酶切鉴定证实pE TXB2重组质粒含有茁2毒素基因而且阅读框架和基因序列正确。同时,采用乳糖诱导剂通过改变培养基的pH值、培养温度、诱导剂的加入量及诱导时间四个方面对茁2毒素基因表达进行调控,从而为茁2毒素基因工程菌的工业化生产提供了可靠的试验数据。In this research we identified the recombinant plasmid pE TXB2 that contains the beta2-toxin gene by using restriction endonucleases digestion and detected expressional level of the beta2-toxin gene under the different culture conditions by SDS-PAGE.The identification of endonucleases digestion confirmed that the recombinant expression plasmid pE TXB2 contains beta2-toxin gene having right open reading frame gene sequence.The lactose was adopted as inducer in this experiment and the recombinant BL21（DE3）（pE TXB2） strains were optimized by changing pH of medium,temperature,amount of inducer loading and inducing time.This research may provide experimental data of the genetically engineered Clostridium perfringens type C for producing beta2-toxin in industrial production.

关 键 词：C型产气荚膜梭菌 茁β2毒素基因 乳糖诱导剂 优化表达 

分 类 号：Q78[生物学—分子生物学]