电击死大鼠心脏超微结构及HSP70、HIF-1α表达变化  

Cardiac Ultrastructure and Changes of HSP70 and HIF-1α Expression in Electric Shock Death Rats

作　　者：张国忠[1] 李瑞利[1] 冯国伟[1] 毕海涛[1] 王松军[1] 丛斌[1] 左敏[1] 

机构地区：[1]河北医科大学法医学系,河北石家庄050017

出　　处：《法医学杂志》2015年第4期262-265,共4页Journal of Forensic Medicine

基　　金：河北省科技支撑计划项目(142777108D)

摘　　要：目的观察电击死大鼠心脏超微结构及热休克蛋白70(heat shock protein 70,HSP70)、低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)的表达变化,探讨其是否能作为电击死法医学鉴定的依据。方法将72只SD大鼠随机分为电击死组、死后电击组和对照组。通过透射电镜观察电击死大鼠心肌超微结构的改变,并采用免疫组织化学技术观测心肌HSP70和HIF-1α的表达变化。结果电击大鼠心肌细胞肌原纤维断裂,线粒体嵴和膜溶解消失,Z线、M线排列紊乱。HSP70和HIF-1α在电击死组呈强阳性表达,与死后电击组、对照组比较差异有统计学意义(P<0.05)。结论 HSP70和HIF-1α在电击死组表达明显上调,有望作为电击死的诊断参考指标。Objective T o observe cardiac ultrastructure and the expression of heat shock protein 70 （H SP70） and hypoxia inducible factor-1α （H IF-1α） in electric shock death rats and to explore the application of these indexes as the basis of m edical identification in electric shock death. Methods Seventy-tw o SD rats w ere random ly divided into electric shock death group, postm ortem electric shock group and the control group. T he changes of m yocardial ultrastructure w ere observed by transm ission electron m icro-scope, and the expressions of m yocardial H SP70 and H IF-1α w ere observed by im m unohistochem ical technology. Results M yocardial m yofibril fracture, m itochondrial cristae and m em brane dissolution, and disordered arrangem ent of Z lines and M lines w ere observed in electric shock rats. H SP70 and H IF-1αw ere strong positive expressions in the electric shock death group, significantly com pared w ith the con-trol and postm ortem electric shock groups （P〈0.05）. Conclusion T he expressions of H SP70 and H IF-1αw ere obviously increased in electric shock death group, w hich m ay be used as the diagnostic indicator of electric shock death.

关 键 词：法医病理学 电击伤 心脏 热休克蛋白70 低氧诱导因子-1Α 大鼠 

分 类 号：DF795.1[医药卫生—法医学]