响应面试验优化胡萝卜浆复合酶解工艺  

Optimization of Enzymatic Hydrolysis of Carrot Pulp with Cellulase and Pectinase for Increased Juice Yield by Response Surface Methodology

作　　者：赵能[1] 罗安伟[1] 姚婕[1] 牛远洋 李琛[1] 刘焕军[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品科学》2015年第16期83-87,共5页Food Science

摘　　要：为优化胡萝卜制汁工艺,以出汁率为指标,通过单因素试验研究纤维素酶-果胶酶配比、酶解时间、酶解温度对胡萝卜出汁率的影响,再通过Box-Behnken试验法与响应面分析法,研究各自变量及其交互作用对胡萝卜出汁率的影响,建立了二次多项式回归预测模型。结果表明:复合酶酶解胡萝卜浆的最佳条件为纤维素酶-果胶酶配比2.2∶10(g/g)、复合酶添加量0.3%、酶解时间1.94 h、酶解温度41.67℃。在此酶解条件下,胡萝卜出汁率为(79.36±0.23)%,与响应面预测值79.06%拟合性较好,对实际生产有一定指导意义。The aim of this work was to optimize the enzymatic hydrolysis of carrot pulp with cellulase and pectinase for enhanced juice yield by response surface methodology（RSM）. The one-factor-at-a-time method was adopted to investigate the effect of cellulase to pectinase ratio, hydrolysis temperature and time on juice yield. The interactions of the three independent variables were analyzed using Box-Behnken design and response surface analysis. As a result, a quadratic polynomial regression model was established. The optimal hydrolysis conditions for producing carrot juice were found to be enzymatic hydrolysis with 0.3% of a mixture of cellulase and pectinase（2.2：10, g/g） for 1.94 h at 41.67 ℃. Experiments carried out under these conditions gave a juice yield of（79.36 ± 0.23）%, was agreeing wit the predicted value（79.06%）.

关 键 词：胡萝卜 复合酶 酶解 出汁率 响应面分析法 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]