HPLC法测定阿巴卡韦双夫定片中的含量  

Determination of Compound Abacavir Sulfate,Lamivudine and Zidovudine Tablets by HPLC

作　　者：杨兴明 陈艳霞 

机构地区：[1]武汉药品医疗器械检验所,武汉430075

出　　处：《中国药师》2015年第9期1615-1616,共2页China Pharmacist

摘　　要：目的：建立HPLC法测定阿巴卡韦双夫定片中阿巴卡韦、拉米夫定、齐多夫定的含量。方法：采用Hypersil C18（200mm×4.6mm,5μm）色谱柱,以甲醇-庚烷磺酸钠溶液（取庚烷磺酸钠2.02 g,加三乙胺5 ml,加水至700 ml,用磷酸调p H至3.9）（30∶70）为流动相,流速为1.0 ml·min-1,检测波长270 nm。结果：拉米夫定、齐多夫定、阿巴卡韦分别在0.08～1.60μg（r=0.999 4）,0.24～4.80μg（r=0.999 9）,0.24～4.80μg（r=0.999 9）范围内线性关系良好,平均回收率分别为99.6%（RSD=0.9%）,99.7%（RSD=0.9%）,99.9%（RSD=1.0%）。结论：本方法简便、可靠、准确,可用于同时测定三组分含量。Objective： To establish an HpLC method for the determination of abacavir, lamivudine and zidovudine in compound abacavir sulfate, lamivudine and zidovudinezivir tablets. Methods： Using a Hypersil C18 （200mm · 4.6mm,5 μm）column, the mobile phase was methanol-sodium heptanesulfonate solution （2.02g sodium heptanesulfonate and 5 ml triethylamine were dissolved in 700 ml water, and then the pH value was ajustal to 3.9 by phosphoric acid） （30：70） at a flow rate of 1.0 ml · min-1 , and the detection wavelength was 270 nm. Results： The calibration curves were linear within the range of 0.08-1.60 μg（ r = 0.999 4） for lamivudine, 0.24-4.80 μg（ r = 0. 999 9） for zidovudine, and 0.24-4.80 ug（ r = 0.999 9） for abacavir. The average recovery was 99.6% （RSD = 0.9% ） for lamivudine, 99.7% （RSD =0.9% ） for zidovudine and 99.9＇% （RSD = 1.0% ） for abacavir. Conclusion： The method is simple, quick and accurate, which can be used for the simultaneous determination for abacavir, lamivudine and zidovudine in com- pound abacavir sulfate, lamivudine and zidovudinezivir tablets.

关 键 词：高效液相色谱法 含量测定 阿巴卡韦 拉米夫定 齐多夫定 

分 类 号：R927.2[医药卫生—药学]