唑来膦酸抑制骨质疏松骨髓脂肪增多  

A single dose of zoledronic acid reverses the marrow adiposity of estrogen deficiency-induced osteoporosis

作　　者：徐铮[1] 顾昊[1] 李冠武[2] 常时新[2] 周蕾[2] 

机构地区：[1]上海市闵行区莘庄社区卫生服务中心,上海201199 [2]上海中医药大学附属岳阳中西医结合医院医学影像科,上海200437

出　　处：《中国骨质疏松杂志》2015年第8期914-917,共4页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金项目(81202809);上海市闵行区卫生局资助(2013MW71)

摘　　要：目的探讨唑来膦酸干预原发性骨质疏松(OP)模型鼠后骨髓脂肪含量变化规律。方法 27只3月龄雌性SD大鼠随机均分为假手术组(SHAM)、去卵巢组(OVX)及OVX+唑来膦酸干预组(ZOL)。分别在术后0、6、12周行MRI水-脂分离成像及BMD扫描,获取骨髓脂肪分数(MFF)及BMD。实验结束行骨髓脂肪细胞定量分析。结果 OVX组MFF呈时序性增高而BMD呈时序性降低。第6、12周时,OVX组MFF较基线时分别增高40.0%、69.4%(P<0.001)。第6周始组间MFF有显著性差异,而组间BMD至第12周时才有统计学差异(P<0.001)。OVX组骨髓脂肪细胞密度、直径及面积百分比较SHAM组分别增高57.3%、29.5%、163%,较ZOL分别增高65%、22.2%、144%(P<0.001)。ZOL组MFF及脂肪细胞定量参数与SHAM组无明显差异。结论 ZOL保护骨受损同时,可能通过抑制骨髓脂肪含量而起作用。Objective To explore the effect of zoledronic acid（ ZA） in restoring marrowadipogenesis in estrogen-deficient rats.Methods Twenty-seven female SD rats were randomly divided equally into SHAM,ovariectomized（ OVX）,and OVX ＋ ZOL groups. Marrowfat fraction（ MFF） and bone mineral density（ BMD） were measured at 0,6,and 12 weeks. Marrowadipocytes were quantitatively evaluated by histopathology. Results OVX rats exhibited time-depend increase in MFF but decrease in BMD.The increase of MFF above baseline was 40. 0% at week 6 and 69. 4% at week 12,respectively（ P 0. 001）. Compared with SHAMrats,MFF in OVX rats markedly elevated at week 6 but a reduction in BMD was first detected at week 12（ P 0. 001）. The adipocyte density,diameter,and volume in OVX rats increased by 57. 3%,29. 5%,and 163%,respectively,compared with those in SHAMrats,and by 65%,22. 2%,and 144%,respectively,compared with those in ZOL rats（ P 0. 001）. MFF and adipocyte parameters were not different between ZOL rats and SHAMrats. Conclusion A single dose of ZOL is able to reverse the marrowadiposity of OVX-induced osteoporosis.

关 键 词：骨质疏松 骨密度 骨髓脂肪 水-脂分离 

分 类 号：R589.5[医药卫生—内分泌]