睾丸与附睾精子DNA完整率及行ICSI结局的对比研究  

Testicular and epididymal sperm comparison of DNA integrity rate and ICSI outcome

作　　者：何泳志 李大文[2] 黄悦悦[2] 肖鑫[1] 毛献宝[2] 龚国通 

机构地区：[1]广西中医药大学,南宁530001 [2]广西壮族自治区人民医院生殖医学与遗传中心,南宁530021

出　　处：《重庆医学》2015年第25期3531-3533,共3页Chongqing medicine

基　　金：广西壮族自治区卫生厅自筹经费科研课题(Z2014191);广西医疗卫生适宜技术研究与开发课题(S201421-03)

摘　　要：目的对比分析睾丸与附睾精子行卵胞浆内单精子注射术(ICSI)的结局及精子DNA完整率(用精子DNA断裂指数DFI表示)的差异。方法对来该院生殖中心就诊的183例梗阻性无精子症患者行ICSI,将患者根据取精途径的不同分为两组,其中经皮附睾穿刺抽吸术(PESA)取精80个周期,经睾丸穿刺抽吸术(TESA)取精103个周期,对比两组精子DNA完整率及行ICSI的结局。结果两种方法精子DNA完整率和行ICSI时受精率、卵裂率、优胚率、妊娠率比较,差异无统计学意义(P>0.05)。结论睾丸及附睾精子的DNA完整率及行ICSI结局无显著差异,临床医生可根据个人经验或患者意愿选取精子行ICSI助孕。Objective To comparative analysis the intracytoplasmic sperm injection （ICSI） result and rate of sperm DNA integrity （DNA fragmentation index,DFI） about testicular and epididymis sperm. Methods Totally 183 obstructed azoospermia patients were choosed to use ICSI. 80 cycles by PESA and 103 cycles by TESA, compared two groups of sperm DNA integrity rate and ICSI outcome. Results Sperm DNA integrity rate, fertilization rate, cleavage rate, good-qualityembryo rate and pregnancy rate compared with no difference by ICSI（P〉0.05）. Conclusion DNA integrity rate and ICSI outcomes of the testis and epididymis sperm have no significant differences,clinicians can be based on personal experiences or patients,wills to select sperm for ICSI.

关 键 词：睾丸精子抽吸术 经皮附睾精子抽吸术 梗阻性无精子症 卵胞浆内单精子注射术 精子DNA完整率 

分 类 号：R698.2[医药卫生—泌尿科学]