低分子肝素对哮喘小鼠气道黏液高分泌和炎症反应的影响  

Effect of low molecular weight heparin on mucus hypersecretion and airway inflammation in asthmatic mice

作　　者：强丽霞[1] 赵婷婷[1] 金寿德[1] 

机构地区：[1]哈尔滨医科大学附属第四医院呼吸内科,黑龙江哈尔滨150001

出　　处：《临床肺科杂志》2015年第10期1797-1801,共5页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨低分子肝素对哮喘小鼠气道黏液高分泌和炎症反应的影响。方法 30只健康雄性C57BL/6小鼠,按随机数字表法分为正常对照组、哮喘组和低分子肝素干预组,每组10只,检测三组小鼠气道上皮细胞黏液分泌量、气道内炎症细胞浸润、黏蛋白基因MUC5AC的表达、细胞因子IL-13、IL-4和IL-5的表达。结果 (1)哮喘小鼠气道黏液细胞化生、黏液过度分泌,气道内炎症细胞浸润增多,经鼻腔滴入低分子肝素后,黏液过度分泌受抑制,气道内炎症细胞浸润减少。(2)哮喘组小鼠气道黏液细胞MUC5AC mRNA相对表达量(26.43±0.47)及气道内炎症因子IL-13[(16.03±0.53)ng/ml]、IL-4[(603.68±50.32)pg/ml]和IL-5[(1308.80±224.84)pg/ml]表达的水平均高于正常组[(1.17±0.18)ng/ml、(17.56±3.01)pg/ml和(33.09±5.92)pg/ml];经鼻腔滴入低分子肝素后,上述指标[16.26±2.07,(11.50±0.89)ng/ml,(409.56±46.25)pg/ml,(874.65±32.833)pg/ml]均下降,P<0.05,差异有统计学意义。结论低分子肝素抑制哮喘小鼠气道黏液高分泌和气道炎症反应。经鼻滴入的低分子肝素可能为哮喘过敏性炎症的治疗提供新的方法。Objective To investigate the effect of low molecular weight heparin on mucus hypersecretion and airway inflammation in asthmatic mice. Methods 30 healthy male C57BL/6 mice were randomly divided into the control group, the asthmatic group and the low molecular weight heparin group. The mucus production, inflammatory cells, MUC5AC, and the levels of cytokines such as IL-13, IL-4 and IL-5 were examined in the three groups. Re- suits （ 1 ） Their mucous cells manufactured, slime over secreted, and inflammatory cell infiltration increased before treatment. After the treatment, slime secretion inhibited and inflammatory cell infiltration decreased. （2） The ex- pression of MUCSAC mRNA in the airway mucous cells, IL-13, IL-4 and IL-5 in the asthmatic group was （26. 43 ± 0. 47） ng/ml, （ 16. 03 ±0. 53） ng/ml, （603.68±50. 32） pg/ml and （ 1308.80±224. 84） pg/ml, which were all significantly higher than those in the control group [ （ 1.17 ± 0. 18） ng/ml, （ 17.56±3.01 ） pg/ml and （33.09 ±5. 92） pg/ml] , and all of them decreased to （16. 26±2. 07） ng/ml, （11.50 ±0. 89） ng/ml, （409. 56±46. 25） pg/ml and （ 874. 65± 32. 833 ） pg/ml respectively after treating with low molecular weight heparin （ P 〈 0. 05 ）. Conclusion Low molecular weight heparin could inhibit mucus hypersecretion and airway inflammation in asthmatic mice. Intranasal instillation with low molecular weight heparin might be a novel approach for asthma treatment.

关 键 词：低分子肝素 黏液高分泌 炎症 哮喘 

分 类 号：R562.25[医药卫生—呼吸系统]