Application of gastric perfusion intralipid for formulating rabbit's hyperlipidemia models  

作　　者：马建林 周燕 马立宁 张家明 李大主 刘时武 李斌 万妮娜 

机构地区：[1]Department of Cardiology, Hainan People's Provincial Hospital [2]Institute of Cardiovascular Disease,Tongji Medical College, Huazhong University of Science and Techology

出　　处：《South China Journal of Cardiology》2015年第2期130-136,共7页岭南心血管病杂志（英文版）

基　　金：supported by Science and Technology Planning Project of Hainan Province(No.20158328)

摘　　要：Background The commonly used method so far for formulating hyperlipidemia animal models is to feed the animals with a high fat diet. Owning to influences of animal feeding, dosage and cycle, feeding with a high fat diet has resulted in an unstable quality. It is unknown whether gastric perfusion of intralipid can establish a better hyperlipidemia model in rabbits. Methods Twenty rabbits were randomly selected and subjected to gastric intralipid perfusion for consecutive 2 weeks or to feed with common high cholesterol feed for 4 weeks. The levels of blood serum total cholesterol （TC）, triglyceride （TG）, apolipoprotein A1 （APOA1）, APOB, blood plasma thromboxane B2 （TXB2）, 6-Keto-prostaglandin F1α （6-Keto-PGF1α）, endotheline （ET）, erythrocyte membrane cholesterol （M-Tc）, membrane fluidity （M-Flu）, erythrocyte malondialdehyde （E-MDA）, erythrocyte superoxide dismutase （E-SOD） before and after the experiment. In the end of the experiment, the aortae were removed from all the animals for light and electron microscopic examinations. Results The results showed that in both groups, the levels of blood serum TC, TG, APOB, blood plasma TXB2, ET, erythrocyte M-Flu and E-MDA were significantly increased. Blood serum APOA1, blood plasma 6-Keto-PGF1α and E-SOD were significantly decreased. But, erythrocyte M-TC had no significantly changed. The indexes of the two groups were no obvious difference before and after the experiment. Light and electron microscopic examination showed that there were early changes of early atherosclosis in the two groups of animal, in accordance with the characteristics of experimental animal hyperlipidemia. Conclusion The results suggest that gastric perfusion of intralipid can produce hyperlipidemia in rabbits faster than the commonly used model developed by feeding high cholesterol diet.Background The commonly used method so far for formulating hyperlipidemia animal models is to feed the animals with a high fat diet. Owning to influences of animal feeding, dosage and cycle, feeding with a high fat diet has resulted in an unstable quality. It is unknown whether gastric perfusion of intralipid can establish a better hyperlipidemia model in rabbits. Methods Twenty rabbits were randomly selected and subjected to gastric intralipid perfusion for consecutive 2 weeks or to feed with common high cholesterol feed for 4 weeks. The levels of blood serum total cholesterol （TC）, triglyceride （TG）, apolipoprotein A1 （APOA1）, APOB, blood plasma thromboxane B2 （TXB2）, 6-Keto-prostaglandin F1α （6-Keto-PGF1α）, endotheline （ET）, erythrocyte membrane cholesterol （M-Tc）, membrane fluidity （M-Flu）, erythrocyte malondialdehyde （E-MDA）, erythrocyte superoxide dismutase （E-SOD） before and after the experiment. In the end of the experiment, the aortae were removed from all the animals for light and electron microscopic examinations. Results The results showed that in both groups, the levels of blood serum TC, TG, APOB, blood plasma TXB2, ET, erythrocyte M-Flu and E-MDA were significantly increased. Blood serum APOA1, blood plasma 6-Keto-PGF1α and E-SOD were significantly decreased. But, erythrocyte M-TC had no significantly changed. The indexes of the two groups were no obvious difference before and after the experiment. Light and electron microscopic examination showed that there were early changes of early atherosclosis in the two groups of animal, in accordance with the characteristics of experimental animal hyperlipidemia. Conclusion The results suggest that gastric perfusion of intralipid can produce hyperlipidemia in rabbits faster than the commonly used model developed by feeding high cholesterol diet.

关 键 词：hyperlipidemia model INTRALIPID gastric perfusion RABBITS 

分 类 号：R589.2[医药卫生—内分泌] R-332[医药卫生—内科学]