羟苯磺酸钙对对比剂肾病大鼠肾的保护作用  

Protective effects of calcium dobesilate on Contrast-induced nephropathy in rats

作　　者：于芬芬[1] 季文萱[1] 单文红[1] 孙艳[1] 刘桂美[1] 黄俊彦[1] 

机构地区：[1]青岛大学医学院第二附属医院肾内科,山东青岛266042

出　　处：《中国介入心脏病学杂志》2015年第8期454-458,共5页Chinese Journal of Interventional Cardiology

基　　金：青岛医疗卫生优秀人才培养项目(青卫科教字【2014】8号)

摘　　要：目的探讨羟苯磺酸钙对对比剂肾病(CIN)模型大鼠的影响。方法将84只大鼠随机分为3组:假手术组(A组)、模型组(B组)和羟苯磺酸钙干预组(C组)。B组和C组分别注射吲哚美辛(INDO)、N-硝基-L-精氨酸甲酯(L-NAME)和76%泛影葡胺建立CIN大鼠模型;A组注射等量INDO+L-NAME+生理盐水;C组于造模前3 d及造模当天给予羟苯磺酸钙[100 mg/(kg·d)]灌胃,A组及B组给予等量生理盐水灌胃。于造模后24 h、48 h、72 h分别处死大鼠(每组7只),取血和肾组织,测定血尿素氮(BUN)、肌酸酐(Scr),HE染色观察肾病理改变;ELISA法检测血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的含量;TUNEL染色检测肾细胞凋亡。结果 B组和C组48 h血清BUN、Scr、MDA表达高于A组,但C组较B组显著降低(P<0.05);B组48 h血清SOD、GSH-Px表达较A组显著降低,C组SOD、GSH-Px表达显著高于B组(P<0.05),但仍低于A组;C组相同时间段病理改变较B组显著减轻,但仍重于A组;造模后48 h C组细胞凋亡较B组显著减轻(P<0.05),但仍重于A组。结论氧化应激和细胞凋亡可能参与大鼠CIN的发生发展,而羟苯磺酸钙可能通过减轻CIN大鼠氧化应激及细胞凋亡发挥保护作用。Objective To investigate the effects of calcium dobesilate on the rats with Contrastinduced nephropathy（ CIN）. Methods 84 Wistar rats were randomly divided into 3 groups： Shamoperated group（ group A）,CIN group（ group B） and calcium dobesilate treated group（ group C）. Rats in group B and group C were given indometacin,L-NAME,76% Urografin in turn by intravenous injection to establish CIN rats model,while rats in group A received equivalent volume of normal saline instead of 76%Urografin. Rats in group C were given calcium dobesilate [100 mg /（ kg·d） ]by gavage from 3 days before the operation until they were executed,while group A and group B were given an equal volume of normal saline. At different time points of 24 h,48 h and 72 h after injection of contrast media,the rats were sacrified for tissue and blood collection. Blood urea nitrogen（ BUN） and serum creatinine（ Scr） were detected and pathological changes of kidney were evaluated by HE staining. The content of malondialdehyde（ MDA）,superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-Px） was detected by ELISA.Apoptosis of tubular cells was detected by TUNEL staining. Results BUN,Scr and the content of MDA in group B and group C were higher than those in group A at 48 h afer modeling,and those in group C were significantly lower than in group B（ P〈0. 05）. The content of SOD and GSH-Px in Group B were significantly lower compared with group A at 48 h afer modeling,and those in group C were significantly higher compared with group B（ P〈0. 05）,but still lower than those in group A. At the same time point,pathological changes of the kidney in group C were significantly better compared with group B,but still more serious than those in group A. Apoptosis of the kidney in group C was significantly decreased compared with group B at 48 h afer modeling,but still more serious than that in group A. Conclusions Oxidative stress and apoptosis have a closed relationship with CIN. Calcium dobesilate can protect C

关 键 词：对比剂肾病 氧化应激 细胞凋亡 羟苯磺酸钙 

分 类 号：R692[医药卫生—泌尿科学]