仁用杏花芽3个发育时期数字基因表达谱分析  

The Analysis of Digital Gene Expression About Kernel Apricot Flower Buds at Three Development Stages

作　　者：宋猜 乌云塔娜[2] 李慧[1] 许靖诗 刘汝卿 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,长沙410004 [2]中国林业科学研究院经济林研究开发中心,郑州450003 [3]北海市林业技术推广站,广西北海536000 [4]怀化市林业调查设计院,湖南怀化418000

出　　处：《园艺学报》2015年第8期1559-1568,共10页Acta Horticulturae Sinica

基　　金：‘十二五’国家科技支撑计划项目(2013BAD14B02)

摘　　要：以仁用杏‘优一’为试材,运用转录组、数字基因表达谱技术,研究了仁用杏花芽萌动期前后相关基因的表达规律。结果发现,仁用杏花芽在花芽萌动期前后进行着各种旺盛的生物合成和代谢活动,且4个开花调控途径(光周期途径、春化途径、自主途径、GA调控途径)均已启动,光周期途径在花芽萌动时发挥重要作用,涉及到了74条基因,其中有38条差异基因上调表达,29条下调表达;GA途径在花芽萌动期对花芽的生长发育调控表现为抑制作用,涉及到了60条基因,其中有13条差异基因上调,9条差异基因下调;其他两条途径于花芽萌动前作用,其中春化途径涉及到了23个基因,其中有4条差异基因上调,15条差异基因下调;自主途径涉及到了24条基因,其中有3条差异基因上调,11条差异基因下调。本研究通过数字基因表达谱分析,初步了解仁用杏花芽萌动前后的网络途径,为后期对仁用杏花芽相关开花基因的研究、探明仁用杏早花的分子机理及通过分子育种方法培育仁用杏晚花品种提供坚实的理论依据。Experiment studied the pattern of related gene expression information around the sprouting period with flower buds of kernel apricot（Armeniaca vulgaris Lam.）‘Youyi＇,applied the transcriptome and digital gene expression profiling technology. The results found that there were many kinds of exuberant biosynthesis and metabolic activity of kernel apricot flower buds around the sprouting period,and four flowering regulation pathways（photoperiod pathway,verbalization pathway,autonomous pathway and GA-dependent pathway）had already been started,and meantime,photoperiod pathway played a significant role,this process involves 74 genes of which 38 up-regulated expression and 29 down-regulated expression;GA-dependent pathway indicating a inhibiting effect in the sprouting period for controlling the development of flower buds,this process involves 38 genes of which 13 up-regulated expression and 9 down-regulated expression. Verbalization pathway and autonomous pathway were effected before the sprouting period. Verbalization pathway has 4 up-regulated expression genes and 15 down-regulated expression genes,autonomous pathway involves 24 genes of which 3 up-regulated expression and 11 down-regulated expression. By analyzing of digital gene expression,we have a preliminary understanding of network way about kernel apricot around the sprouting period, the information could provide theoretical basis for the research of flowering genes involved in kernel apricot blossom,exploring on the molecular mechanism of early blooming and breeding new varieties by molecular method.

关 键 词：仁用杏 花芽 转录组 数字基因表达谱 成花调控途径 

分 类 号：S662.9[农业科学—果树学]