高铜日粮对肉鸡肝脏Trx2蛋白表达的影响  

Effects of High Level Copper Diet on Expression of Trx2 Protein in Broiler Liver

作　　者：王荣梅[1] 赵翠燕[1] 闫文龙[1] 陈立军 

机构地区：[1]韶关学院英东农业科学与工程学院,广东韶关512005 [2]广西出入境检验检疫局检验检疫技术中心,广西南宁530021

出　　处：《中国兽医杂志》2015年第7期11-13,共3页Chinese Journal of Veterinary Medicine

基　　金：2014年广西自然科学基金项目(2014GXNSFBA118132);国家自然科学基金(30871900)

摘　　要：将200羽1日龄Cobb商品代肉鸡随机分为4组,各组日粮中铜含量分别为:对照组(Ⅰ组)11 mg/kg、试验Ⅱ组110 mg/kg、试验Ⅲ组330 mg/kg、试验Ⅳ组550 mg/kg。饲养至10、30、50 d时每组各取5只鸡用于采集肝脏样品,提取肝脏总RNA用RT-PCR法测定Trx2基因m RNA在肝脏中的表达量,用蛋白质印迹法检测Trx2蛋白表达量。结果显示:日粮铜含量为11 mg/kg、110 mg/kg和330 mg/kg时,随着试验时间的延长,肝脏Trx2基因表达升高,但差异不显著(P>0.05),而铜含量为550 mg/kg时,Trx2基因表达量降低且50 d时差异显著(P<0.05);随着试验时间的延长,各试验组的Trx2蛋白表达量均升高,且330 mg/kg和550 mg/kg组在50 d时差异显著(P<0.05)。试验表明,饲喂高铜日粮可导致线粒体型硫氧还蛋白2(Trx2)mRNA在肝脏中的表达量先升高后降低,蛋白表达量升高。The experiment was conducted with two hundred healthy 1 day-old Cobb broilers which were randomly divided into four groups based on diets as follows：control group（Cu 11mg/kg as the group Ⅰ）and high copper groups（Cu content 110,330 and 550mg/kg,named group Ⅱ,Ⅲ and Ⅳ）. The liver was taken from broiler in each group on the 10 th,30 th and 50 th days respectively,the testing material was liver mitochondria which was extracted extraction from the liver,and the expression of the liver Trx2 m RNA and protein were determined by the method of semi-quantification and reverse transcription polymerase chain reaction（RT-PCR）,Western blot.The results showed that Trx2 m RNA expression of groupⅠ,Ⅱ,Ⅲ increased with time,but showed no significant difference（P0.05）,that of groupⅣ decreased obviously on day 50.Trx2 protein expression of all groups increased with time,that of group Ⅲ,Ⅳ significantly increased（P0.05）on day 50. The experimental results indicated that fed high copper diets could increase Trx2 m RNA expression first and decrease after on 50 days,and increase Trx2 protein expression during the experimental period.

关 键 词：铜 肉鸡 肝脏 Trx2 蛋白表达 

分 类 号：S856.4[农业科学—临床兽医学]