HBV相关慢加亚急性肝衰竭患者外周血Th1、Th2、Th17的表达和意义  

The Changes and Clinical Signifi cance of T Helper Lymphocyte Subsets of the Patients with HBV-Associated Subacute-On-Chronic Liver Failure

作　　者：顾静[1] 甘建和[1] 陈丽[1] 王艳[1] 姚运海[1] 

机构地区：[1]苏州大学附属第一医院,江苏苏州215006

出　　处：《中国血液流变学杂志》2015年第1期7-9,共3页Chinese Journal of Hemorheology

摘　　要：目的：探讨HBV相关慢加亚急性肝衰竭（HBV-SACLF）患者外周血Th1、Th2、Th17的表达水平及意义。方法运用流式细胞技术检测38例HBV-SACLF患者、20例慢性乙型病毒性肝炎（CHB）患者、20名健康体检者（对照组）外周血中Th1、Th2、Th17的水平；动态观察HBV-SACLF患者早、中、晚期Th1、Th2、Th17的变化趋势。结果1.Th1在HBV-SACLF组与CHB组及对照组比较，明显升高（P1=0.04，P2=0.032）。Th2在HBV-SACLF组与CHB组及对照组比较，明显下降（P1=0.007，P2=0.041）。Th17在HBV-SACLF组与CHB组及对照组比较，明显升高（P1=0.003，P2＜0.001）。2.HBV-SACLF患者早期与中期，中期与晚期相比各细胞水平变化趋势：Th1逐渐降低（P1=0.001，P2=0.004）。Th2逐渐升高（P1=0.003，P2=0.048）。Th17逐渐升高（P1=0.002，P2=0.007）。结论 Th1、Th2、Th17的紊乱是导致HBV-SACLF发生、发展的关键因素，通过检测其在HBV-SACLF患者早、中、晚期的动态变化可以辅助判断机体的免疫状态，为临床的免疫调控治疗提供理论依据。Objective To investigate the dynamic change and clinical significance of Th1, Th2 and Th17 in peripheral blood of the patients with SACLF during different periods.Methods We detected the ratio of Th1, Th2 and Th17 from peripheral blood cells in 38 HBV-SACLF patients, 20 CHB patients and 20 health adults byfl uorescence-activated cell sorter; compared the above indicators among the three groups; observed dynamic changes of Th1, Th2 and Th17 during the nonage, metaphase and advanced stage of HBV-SACLF.Results 1.The ratios of Th1 in HBV-SACLF group were significantly higher than CHB group and control group （P1=0.04, P2=0.032）. The ratios of Th2 in HBV-SACLF group were signifi cantly lower than CHB group and control group （P1=0.007,P2=0.041）. The ratios of Th17 in HBV-SACLF group were significantly higher than CHB group and control group （P1=0.003,P2〈0.001）. 2.The ratios of Th1, Th2 and Th17 cells in the nonage, metaphase and advanced stage of 38 HBV-SACLF patients were as follows： The ratios of Th1 decreased gradually. There were also significant differences among the three periods （P1=0.001,P2=0.004）. The ratios of Th2 increased gradually. There were also signifi cant differences among the three periods （P1=0.003,P2=0.048）. The ratios of Th17 increased gradually. There were also signifi cant differences among the three periods （P1=0.002,P2=0.007）. Conclusion The disorders of Th1, Th2 and Th17 were key factors in the development of HBV-SACLF. By detecting their dynamic changes in HBV-SACLF, we can judge patients＇ immune status and offer theoretical basis for the clinical treatment of immune regulation.

关 键 词：乙型肝炎病毒 慢加亚急性肝衰竭 TH1 TH2 TH17 TH1 TH2 TH17 

分 类 号：R512.62[医药卫生—内科学]