补肾散结方对肺癌骨转移小鼠PTHrP及TGF-β_1表达的影响  

Effects of “Bushen Sanjie Decoction”on PTHrP and TGF-β_1 in Lung Cancer Mice with Bone Metastasis

作　　者：赵凌艳[1] 徐祖红 李炜[1] 张炜[1] 王中奇[1] 

机构地区：[1]上海中医药大学附属龙华医院肿瘤科,上海200032

出　　处：《上海中医药大学学报》2015年第4期38-42,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市卫生局中医药科研基金项目(2012J004A)

摘　　要：目的:建立LL/2-Luc-M38小鼠肺癌骨转移模型,探讨补肾散结方对肺癌骨转移及PTHr P、TGF-β1表达的影响。方法:在C57小鼠骨髓腔接种LL/2-Luc-M38肺癌细胞株建立骨转移模型,并随机分为模型组、帕米膦酸钠组、补肾散结组。模型组以生理盐水灌胃,帕米膦酸钠组腹腔注射帕米膦酸钠(0.1 ml/次),补肾散结组小鼠以补肾散结方药液灌胃。观察小鼠X线股骨表现和骨转移细胞IVIS荧光信号,骨肿瘤组织病理,骨组织PTHr P、TGF-β1蛋白表达和mRNA表达情况。结果:模型组见明显溶骨性骨破损、肿瘤细胞密集,补肾散结组和帕米膦酸钠组骨皮质轻度破坏、肿瘤细胞减少;帕米膦酸钠组和补肾散结组骨组织肿瘤细胞荧光信号表达量均低于模型组(P<0.05,P<0.01);帕米膦酸钠组、补肾散结组骨组织PTHr P、TGF-β1mRNA表达均明显低于模型组(P<0.05,P<0.01)。结论:补肾散结方可能通过抑制TGF-β1、PTHr P蛋白和mRNA表达达到抗肿瘤骨转移的作用。Objective： To establish the model of LL/2-Luc-M38 bone metastasis in mice to investigate the effects of ＂Bushen Sanjie Decoction＂ on metastic bone of lung cancer, PTHrP and TGF-β expression. Methods： C57 mice were inoculated with LIM2-Luc-M38 lung carcinoma ceils into bone marrow cavity to build bone metastasis model and then randomized into model group, pamidrenate group and ＂Bushen Sanjie Decoction＂ group. The mice in the control group were fed with normal saline, the mice in pamidronate group were intraperitoneally injected with pamidronate （0.1 ml） for 5times, and the ＂Bushen Sanjie Decoction＂ group were fed with ＂Bushen Sanjie Decoction＂ group. The X-ray performance of bone, the IVIS fluorescence signal of femur bone metastatic cells, the protein expressions and mRNA expressions of PTHrP and TGF-β1 were observed. Results： The mice in model group were shown marked osteolytic bone damage and dense tumor cells, with more serious lesion in model group than in pamidronate group and ＂Bushen Sanjie Decoction＂ group. The expressions of tumor cell fluorescence signal were lower in pamidronate group and ＂Bushen Sanjie Decoction＂ group than in model group （P 〈 0.05, P 〈 0.01 ）. The expressions of protein and mRNA of PTHrP and TGF-β1 were lower in pamidr0nate group and ＂Bushen Sanjie Decoction＂ group than in model group（P 〈0.05, P 〈 0. 01 ）. Conclusion： ＂Bushen Sanjie Decoction＂ may inhibit protein and mRNA expressions of TGF-β1 and PTHrP to suppress tumor bone metastases.

关 键 词：补肾散结方 肺癌 骨转移 甲状旁腺激素相关蛋白 转化生长因子-Β1 小鼠 

分 类 号：R285.5[医药卫生—中药学]