龙胆苦苷对雨蛙素致急性胰腺炎小鼠的治疗作用  

Therapeutical Effect of Gentiopicroside on Caerulein-induced Acute Pancreatitis in Mice

作　　者：顾伟梁[1] 吕见[1] 陈长勋[1] 

机构地区：[1]上海中医药大学中药学院药理教研室,上海201203

出　　处：《上海中医药大学学报》2015年第4期47-50,57,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81202944)

摘　　要：目的:探讨龙胆苦苷对雨蛙素诱导的小鼠急性胰腺炎(acute pancreatitis,AP)的治疗作用。方法:采用腹腔连续注射雨蛙素(每次20μg/kg,共5次),建立小鼠AP模型。龙胆苦苷高、低剂量组(300 mg/kg和150 mg/kg)在开始造模后1、5、9 h腹腔注射给予龙胆苦苷,并于小鼠首次腹腔注射雨蛙素后12 h采集样本。观察血清淀粉酶(AMS)和脂肪酶(Lipase)活性,肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)浓度,小鼠胰腺重量系数,组织含水量和胰腺组织形态学的变化。结果:模型组小鼠血清AMS、Lipase活性,TNF-α和IL-1β浓度以及胰腺重量系数和组织含水量均显著升高(P<0.05,P<0.01);龙胆苦苷高、低剂量组腹腔注射给药可显著减弱血清AMS、Lipase活性,降低血清TNF-α和IL-1β浓度,降低急性胰腺炎小鼠胰腺重量系数和组织含水量(P<0.05,P<0.01),改善胰腺组织病理形态学变化。结论:龙胆苦苷具有良好的抗急性胰腺炎作用;其作用机制可能与保护胰腺细胞、抑制炎症介质的激活和释放有关。Objective： To investigate the effect of gentiopicroside on caerulein-induced acute pancreatitis in mice. Methods： Acute pancreatitis （AP） mice model was made by intraperitoneal infusion of caerulein （20 μg/kg） in mice. Gentiopicroside was given by intraperitoneal injection 1,5 and 9 hours after the beginning of intraperitoneal injection of caerulein in mice in both high dose group（300 mg/kg ） and low dose group（ 150 mg/kg）. Samples were got 12 hours after the first time of intraperitoneal infusion of caerulein in mice. Serum amylase and lipase activity, TNF-α and IL-1β concentrations, the pancreas mass/body mass index, tissue water content and the histopathological changes were determined. Results： Amylase and lipase activity, TNF-α and IL-1 β concentrations, weight coefficient and water content in pancreatic tissue increased significantly（ P 〈 0.05, P 〈 0. 01 ） in the model group, but in both high dose and low dose groups, gentiopicroside markedly reduced the pancreatitis-evoked serum amylase and lipase activity, and decreased TNF-α and IL- 1β concentrations, weight coefficient and water content in pancreatic tissue, and improved histopathological changes in pancreatic tissue. Conclusion： Gentiopicroside has the remarkable function of against acute pancreatitis, and the action mechanism may be related to activation and inhibiting the release of inflammatory mediators.

关 键 词：龙胆苦苷 急性胰腺炎 雨蛙素 炎症 

分 类 号：R285.5[医药卫生—中药学]