不同方法对甜菜叶片基因组DNA提取效果比较与适用性探讨  

Comparison of Different Leaf Genomic DNA Extraction Methods for Their Efficacies and Applicability in Sugarbeet(Beta valgaris L.)

作　　者：王希[1,2,3,4] 陈丽[1,2,3,4] 赵春雷[1,2,3,4] 李彦丽[1,2,3,4] 丁广洲[1,2,3,4] 贾海伦[1,2,3,4] 徐杰[5] 廖鹏[5] 

机构地区：[1]中国农业科学院甜菜研究所,黑龙江哈尔滨150080 [2]黑龙江大学农作物研究院,黑龙江哈尔滨150080 [3]黑龙江省普通高等学校甜菜遗传育种重点实验室,黑龙江哈尔滨150080 [4]中国农业科学院北方糖料作物资源与利用重点开放实验室,黑龙江哈尔滨150080 [5]黑龙江大学研究生学院,黑龙江哈尔滨150080

出　　处：《中国糖料》2015年第5期3-6,共4页Sugar Crops of China

基　　金：现代农业产业技术体系建设专项资金(CARS-210101);黑龙江省教育厅科学技术研究面上项目(12531488);黑龙江大学青年科学基金项目(QL201124)

摘　　要：对比了7种不同原理的提取方法对甜菜叶片基因组DNA的提取效果,从得率、纯度、耗时等多方面分析DNA提取效果,为不同研究目的确定了最适的提取方法。最终确定了SDS法是最适合自甜菜叶片中低成本大量提取DNA的方法,CTAB也基本能满足要求,吸附柱法是最适合高通量提取优质DNA的方法,碱裂解法是最适合样品迅速检测的方法,必要时可改用ROSE法。柠檬酸钠法产物质量不高且操作不够简便,尿素法产物纯度过低且耗时过长,在应用上未见优势。In this study, seven different methods with different working principles were used to extract genomic from sugarbeet （B. vulgaris L） leaves, and were compared for multiple features including yield, purity, time- consuming, cost and so on. Suitable methods were selected for different kind of studies. SDS method is the best method for many studies because of its low cost, high yield and acceptable purity. Absorbing column method prepares DNA with the highest quality and cost less time, so it＇s suitable for higher demanded studies. Alkali lysis method is the fastest method for PCR test. ROSE method can be used instead of alkali lysis method if needed. Carbamide method and sodium citrate method are not suitable for genomic DNA extraction from sugarbeet leaves.

关 键 词：甜菜 基因组DNA 提取方法 

分 类 号：S566.301[农业科学—作物学] Q78[生物学—分子生物学]