碱性成纤维细胞生长因子在老龄大鼠血清及阴茎海绵体中的含量  

Concentration of basic fibroblast growth factor in plasma and cavernous corpus of aged rat

作　　者：杨竣[1] 王涛[1] 张岩[1] 徐华[1] 王少刚[1] 刘继红[1] 叶章群[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《北京大学学报（医学版）》2015年第4期582-585,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(81070484)资助~~

摘　　要：目的:探讨老龄大鼠血清及阴茎海绵体中碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)的含量。方法:12只24月龄SD雄性大鼠作为老龄大鼠组,10只12周龄SD雄性大鼠作为正常对照组。采用电刺激海绵体神经方法评价大鼠阴茎勃起功能,留取标本后ELISA方法检测血清和阴茎海绵体组织中b FGF水平,masson染色方法检测阴茎海绵体组织中平滑肌含量。结果:与正常对照相比,老龄大鼠的勃起功能明显下降(阴茎海绵内压/颈动脉压值在2.5、5.0和7.5 V电压刺激时分别为0.41±0.05 vs.0.70±0.06,0.44±0.04 vs.0.75±0.07和0.51±0.06 vs.0.81±0.04,P<0.05),血清和阴茎海绵体组织中b FGF水平明显降低[分别为(6.43±0.51)vs.(10.53±0.42)μg/L,P<0.05;(598.6±51.7)pg/mg protein vs.(985.8±76.8)pg/mg protein,P<0.05],同时海绵体平滑肌含量也明显减少(大鼠阴茎组织平滑肌与胶原纤维的比值0.038±0.005 vs.0.075±0.006,P﹤0.05)。结论:阴茎海绵体组织中b FGF水平降低可能与海绵体平滑肌含量减少有关。Objective： To investigate the concentration of basic fibroblast growth factor（ b FGF） in aged rat plasma and penile tissues. Methods： Twelve 24-month-old rats and ten 12-week-old rats were selected. We assessed the erectile responses of rats to cavernous nerve electrostimulation. Then the concentrations of b FGF in the rats＇ plasma and penile tissues were detected by ELISA kit and smooth muscle contents in the rats＇ cavernous corpus were evaluated by masson trichrome staining. Results： Compared with the young rats,we found that the erectile function of the aged rats were significantly attenuated（ The Max ICP / MAP in the aged rats were 0. 41 ± 0. 05,0. 44 ± 0. 04 and 0. 51 ± 0. 06 at 2. 5 volts,5. 0 volts and7. 5 volts respectively while the Max ICP / MAP in the normal controls were 0. 70 ± 0. 06,0. 75 ± 0. 07 and 0. 81 ± 0. 04 at 2. 5 volts,5 volts and 7. 5 volts respectively,P〈0. 05）. The concentrations of b FGF[The b FGF levels in plasma and penile tissues in the aged rat were（ 6. 43 ± 0. 51） μg /L and（ 598. 6 ±51. 7） pg / mg protein respectively while the b FGF levels in the normal control were（ 10. 53 ± 0. 42） μg /L and（ 985. 8 ± 76. 8） pg / mg protein]were significantly reduced（ P〈0. 05）. Furthermore,the smooth muscle contents in the aged rats＇ penile tissues（ 0. 038 ± 0. 005） were dramatically decreased compared with the normal control（ 0. 075 ± 0. 006,P ﹤ 0. 05）. Conclusion： The reduced levels of b FGF may be related to the decreased smooth muscle contents in the penile tissues of the aged rats.

关 键 词：勃起功能障碍 成纤维细胞生长因子2 阴茎 血清 大鼠 

分 类 号：R697.1[医药卫生—泌尿科学]