磷脂酰肌醇3-磷酸在乙烯诱导蚕豆保卫细胞H_2O_2和NO产生以及气孔关闭中的作用  

The Roles of Phosphatidylinositol 3-Phosphate in Ethylene-Induced Stomatal Closure and Production of H_2O_2 and NO in Broad Bean Guard Cells

作　　者：高媛[1] 樊彩明 胡洁[1] 贺军民[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《植物生理学报》2015年第8期1299-1306,共8页Plant Physiology Journal

基　　金：国家自然科学基金(31170370);中央高校基本科研业务费项目(GK201401005)

摘　　要：以蚕豆(Vicia faba)叶片下表皮为材料,结合药理学试验、气孔开度分析和激光扫描共聚焦显微镜技术,探讨磷脂酰肌醇3-磷酸(PI3P)在乙烯诱导蚕豆保卫细胞中H2O2和NO产生以及气孔关闭中的作用。结果显示,磷脂酰肌醇3-激酶抑制剂沃曼青霉素(WM)和LY294002(LY)显著抑制乙烯释放剂乙烯利诱导的蚕豆保卫细胞H2O2和NO产生以及气孔关闭,外源H2O2和NO处理能完全逆转WM和LY对乙烯利诱导的气孔关闭的抑制效应。结果暗示,PI3P通过诱导信号分子H2O2和NO的产生参与了乙烯诱导蚕豆气孔关闭的细胞信号转导过程。By pharmacological analysis, stomatal bioassay and laser-scanning confocal microscopy, the roles of phosphatidylinositol 3-phosphate（PI3P） in ethylene-induced hydrogen peroxide（H2O2） and nitric oxide（NO） production in guard cells and stomatal closure were studied in the epidermal strips of abaxial surface of broad bean（Vicia faba） leaves. The results showed that both wortmannin（WM） and LY294002（LY）, inhibitors of phosphatidylinositol 3-kinase, signifi cantly inhibited ethephon（ethylene-releasing compound）-induced production of H2O2 and NO in guard cells and subsequent stomatal closure, while application of exogenous H2O2 and NO completely rescued the inhibitory effect of WM and LY on the ethephon-induced stomatal closure. These results suggest that PI3 P participates in the signal transduction pathway of ethylene-induced stomatal closure via inducing the signaling molecules H2O2 and NO in guard cells of broad bean.

关 键 词：磷脂酰肌醇3-磷酸 一氧化氮 过氧化氢 乙烯 气孔运动 

分 类 号：Q942[生物学—植物学]