印度南瓜果皮和果肉颜色遗传分析及基因定位  

Genetic Analysis and Gene Mapping of Rind and Flesh Color of Cucurbita maxima

作　　者：葛宇[1,2] 李雪[1] 杨晓霞[1] 徐文龙[1] 崔崇士[1] 屈淑平[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]中国热带农业科学院海口实验站,海口570102

出　　处：《西北植物学报》2015年第8期1524-1529,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：公益性行业(农业)科研专项(201303112)

摘　　要：以印度南瓜‘98-2-351’与‘06820-1’杂交构建F2群体,对亲本及各世代群体成熟果实果皮和果肉颜色进行调查、统计分析。结果表明:F2群体中果皮桔红色和灰色的分离比呈3∶1,说明果皮灰色是由单隐性基因控制;F2群体中果肉黄色和白色的分离比呈3∶1,说明果肉白色也是由单隐性基因控制。利用群体分离分析法结合隐性群体分析法,采用SSR分子标记,找到了2个与控制灰色果皮基因位点CmRc紧密连锁的SSR标记(PU078072和PU013839),其连锁遗传距离分别为5.9cM和14.5cM;同时找到了1个与控制白色果肉基因位点CmFc紧密连锁的SSR标记PU132712,其连锁遗传距离为6.7cM。本研究为进一步筛选与控制印度南瓜果皮和果肉颜色基因更加紧密连锁的分子标记及相关基因的精确定位奠定了基础。The F2 population was derived by crossing two diverse Cucurbita maxima lines‘98-2-351＇and‘06820-1＇.Investigation and statistical analyses were conducted on rind and flesh color of mature fruits for parental plants and each generation.The results demonstrated that the orange and grey rind plants in F2 population were fitted for the ratio of 3∶1,indicating that the grey rind was controlled by a single recessive gene;similarly,the yellow and white flesh plants in F2 population were also fitted for the ratio of 3∶1,showing that the white flesh was controlled by a single recessive gene.With bulked segregation analysis and recessive-class approaches,two SSR markers PU078072 and PU013839 were detected to be tightly linked to the locus CmRc at a distance 5.9and 14.5cM,respectively.Meanwhile,one SSR marker PU132712 was founded to be closely linked to the locus CmFc at a distance 6.7cM.This study laid the foundation for further screening the molecular markers closer linked to rind and flesh color genes of C.maximaand locating them accurately.

关 键 词：印度南瓜 果皮颜色 果肉颜色 群体分离分析法 隐性群体分析法 

分 类 号：Q343.17[生物学—遗传学] S642.1[农业科学—蔬菜学]