濒危物种珊瑚菜遗传多样性的ISSR分析  

Genetic Diversity of Glehnia littoralis Populations Revealed by ISSR Molecular Markers

作　　者：王爱兰[1] 王贵琳[1] 李维卫[2] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]鲁东大学学报编辑部,山东烟台264025

出　　处：《西北植物学报》2015年第8期1541-1546,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：山东省自然科学基金(ZR2010CM056)

摘　　要：该研究采用ISSR分子标记对中国10个居群的241个珊瑚菜样本进行了遗传多样性分析。结果显示:8条引物共检测到76条清晰谱带,其中多样性条带64条;POPGENE分析显示,其物种水平多样性条带百分率(PPB)为84.21%,有效等位基因数(Ne)为1.562 8,Shannon多样性指数(I*)为0.866 3,Nei’s遗传多样性指数(h*)为0.342 5,居群间的遗传分化系数(GST)为0.205,基因流(Nm)为1.939 1,表明野生珊瑚菜具有较高的遗传多样性,且大部分遗传多样性存在于居群内;AMOVA分析显示,珊瑚菜居群间遗传分化水平(FST)为0.259 1,也表明珊瑚菜居群内变异大于居群间变异。研究认为,珊瑚菜的濒危原因主要来源于野生生态环境的破坏,应当加强种质资源的保护。In order to formulate conservation strategies of this species,We assessed the genetic diversity of G.littoralis with total 241 samples of 10 populations using molecular marker ISSR analysis.The result revealed that high levels of genetic variations occurred within and among populations（PPB=84.21%,Ne=1.562 8,I＊=0.866 3,h＊=0.342 5）.The genetic differentiation coefficient（GST）among populations was0.205.Analysis of molecular variance（AMOVA）demonstrated that 25.91% of genetic diversity was found among populations,74.09% of genetic diversity was found within populations.The value of gene flow（Nm）was 1.939 1.It is indicated that the endangered status of this species is probably due to destruction of habitats of the wild populations,rather than a loss of the genetic diversity.

关 键 词：珊瑚菜 ISSR 遗传多样性 基因流 

分 类 号：Q789[生物学—分子生物学] Q949.763.3