害蚕螽斯防治饵剂中毒死蜱的高效液相色谱分析方法  

Analysis of Bait for Killing Katydids by HPLC

作　　者：张惠淳[1] 黄均伟[1] 牛雄雷[1] 倪振田[1] 

机构地区：[1]辽宁省蚕业科学研究所,辽宁凤城118100

出　　处：《辽宁农业科学》2015年第4期32-34,共3页Liaoning Agricultural Sciences

基　　金：国家现产业技术体系建设项目(CARS-22-SYZ02);辽宁省科学事业公益研究基金(2012005004)

摘　　要：柞蚕受螽斯危害严重,利用商品化颗粒饵剂防治害蚕螽斯是防治方法的革新。建立了在同一液相色谱条件下测定防治害蚕螽斯饵剂中毒死蜱含量的方法。本方法采用NH2基SPE固相萃取柱滤过不易分离的凡士林、蜂蜡杂质,用C18色谱柱,以乙腈—水—冰乙酸(体积比82∶17.5∶0.5)为流动相,在检测波长290 nm下,外标法对饵剂中毒死蜱进行定量分析。分析结果表明,标准偏差为0.0075,变异系数为1.45%;平均回收率为97.87%;线性相关系数相关系数r=0.9995,是对在线产品检测的理想方法。Katydids caused serious damage to rear of antherae perny. The control of Katydids via tablet bait is the innovation of control method. An HPLC method was developed for the determination of chlorpyrifos in bait for killing Katydids. The method use NH2 based SPE column filtration to remove Vaseline and beeswax impurities,which is not easy to separate conventionally. The HPLC conditions were via C18 column using acetonitrile-water-glacial acetic acid = 82∶ 17. 5∶ 0. 5（ by vol） as mobile phase with UV detection at 290 nm. The results showed that the variation coefficients,standard deviations and average recoveries of chlorpyrifos were0. 0075,1. 45%,97. 87%,respectively,with a linear correlations of 0. 9995.

关 键 词：螽斯 毒死蜱 NH2基SPE柱 高效液相色谱 

分 类 号：O657.7[理学—分析化学] TQ460.72[理学—化学]