日本盆景中截获的短体线虫与根结线虫的鉴定  

Identification of Pratylenchus and Meloidogyne nematodes intercepted in Bonsai from Japan

作　　者：王金成[1] 林宇[1] 郭京泽[1] 顾建锋[2] 陈先锋[2] 魏亚东[1] 

机构地区：[1]天津出入境检验检疫局动植物与食品检测中心,天津300456 [2]宁波出入境检验检疫局

出　　处：《植物检疫》2015年第4期38-42,共5页Plant Quarantine

基　　金：国家质检总局课题(2011IK176);国家科技支撑计划项目(2012BAK11B03);天津局科技项目(2013TK072)

摘　　要：2014年,天津口岸从日本进境盆景中截获3种短体线虫和1种根结线虫。通过形态学和DNA条形码方法将截获的短体线虫鉴定为朱顶红短体线虫(Pratylenchus hippeastri)、伤残短体线虫(P.vulnus)和穿刺短体线虫(P.penetrans)。根据形态学并结合特异PCR和DNA条形码方法,将截获的根结线虫2龄幼虫鉴定为南方根结线虫(Meloidogyne incognita)。朱顶红短体线虫是短体线虫属非中国种,为我国进境植物检疫性有害生物,在挪威槭和榉树盆景中截获该线虫为国内外首次报道。In 2014, three species of Pratylenchus and one species of Meloidogyne were intercepted from imported Japan bonsai in Tianjin seaport. Through comprehensive identification of morphological and DNA Barcoding methods the intercepted Pratylenchus nematodes were identified as Pratylenchus hippeastri, P. vulnus and P. penetrans. Based on morphological, specific-primer PCR and DNA Barcoding methods the intercepted second-stage juveniles of Meloidogyne nematodes were identified as Meloidogyne incognita. P. hippeastri belongs to the non-Chinese species of Pratylenchus and was listed as the quarantine pest of China. This is the first report of intercepting P. hippeastri in Acer platanoides and Zelkova schneideriana from Japan.

关 键 词：朱顶红短体线虫 伤残短体线虫 穿刺短体线虫 南方根结线虫 形态特征 PCR 28Sr RNA-D2/D3区 鉴定 

分 类 号：S41-3[农业科学—植物保护]