细胞毒T淋巴细胞相关抗原4基因多态性与宫颈癌的相关性研究  

The correlation of cytotoxic T lymphocyte antigen 4 polymorphisms with occurrence of cervical cancer

作　　者：王甲甲[1] 王智斌[1] 黄文芳[1] 

机构地区：[1]四川省医学科学研究院.四川省人民医院检验科,四川成都610072

出　　处：《实用医院临床杂志》2015年第5期77-80,共4页Practical Journal of Clinical Medicine

摘　　要：目的探讨细胞毒T淋巴细胞抗原4(cytotoxic T lymphocyte antigen 4,CTLA-4)-318 T/C、CT60 G/A、+49 G/A基因多态性与宫颈癌之间的相关性。方法选取2012年12月至2014年7月292例宫颈癌患者(患者组),及同期355例健康体检者(对照组),采集研究对象外周静脉血5 ml,抽提全血基因组DNA,PCR扩增后直接测序法检测CTLA-4-318 T/C,CT60G/A,+49 G/A基因多态性的分布。结果 Logistic回归分析显示,-318CC和CT60 AA基因型均与宫颈癌发病相关(P<0.05)。与对照组比较,携带CTLA-4-318T、CT60 g等位基因的宫颈癌患者明显增加(均P<0.05);携带CTLA-4+49 g G基因型、+49A等位基因的宫颈癌患者,差异无统计学意义(P>0.05)。结论 CTLA-4-318、CT60基因多态性与宫颈癌发病有关。Objective To explore the correlation between cytotoxic T lymphocyte antigen 4 （CTLA-4）genotypes-318 T/C, CT60 G/A, ＋49 G/A and occurrence of cervical cancer. Methods Two hundred and ninety-two patients with cervical cancer from December 2012 to July 2014 were selected as study group, and 355 cases of healthy controls were recruited as control group. Peripheral blood of each participant was collected and genomic DNA was extracted. The distribution of CTLA-4-318 T/C, CT60 G/A, ^-49 G/A genotypes were detected by direct sequencing. Results The CTLA-4-318 CC and CT60 AA genotypes were correlated with the decrease of cervical cancer risk （P 〈 0. 05 ）. Compared with control group, the frequencies of CTLA-4-318T allele and CT60 g allele carriers were significantly higher in the cancer patients （P 〈 0. 05 ）. No significant differences were found in the genotype and allele frequencies in ＋ 49 G/A between patients and healthy controls（P 〉 0. 05 ）. Conclusion The polymorphisms of CTLA-4-318 and CT60 are correlated with cervical cancer risk.

关 键 词：CTLA-4 基因多态性 宫颈癌 

分 类 号：R446.62[医药卫生—诊断学]