Microbial aerosol characteristics in highly polluted and nearpristine environments featuring different climatic conditions  

作　　者：Kai Wei Yunhao Zheng Jing Li Fangxia Shen Zhuanglei Zou Hanqing Fan Xinyue Li Chang-yu Wu Maosheng Yao 

机构地区：[1]State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University [2]Max-Plank Institute for Chemistry [3]School of Water Resources and Environment, China University of Geosciences [4]Department of Environmental Engineering Sciences,Engineering School of Sustainable Infrastructure and Environment, University of Florida

出　　处：《Science Bulletin》2015年第16期1439-1447,共9页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(21277007,21477003,and 41121004);the Ministry of Science and Technology of China(2015DFG92040,2015CB553401);Ministry of Education(20130001110044)

摘　　要：There is an increasing interest in understanding ambient bioaerosols due to their roles both in health and in climate. Here, we deployed an Ultraviolet Aerodynamic Particle Sizer to monitor viable （fluorescent） bioaerosol concentration levels at city centers （highly polluted） and their corresponding suburbs （near pristine） （total 40 locations） in 11 provinces featuring different climate zones in China between July 16 and 28, 2013. The concentration levels of viable bioaerosol particles （BioPM） of 〉0.5 μm were measured, and corresponding percentages of BioPM% （biological fraction of total PM） and BioPM2.5% （biological fraction of PM2.5） in particulate matter （PM） and BioPM, respectively, were determined. For some key cities, indoor viable bioaerosol levels were also obtained. In addition, bacterial structures of the air samples collected across these monitoring locations were studied using pyrosequencing. BioPM concentration levels ranged from 2.1 ×10^4 to 2.4 × 10^5/m3 for city centers [BioPM% = 6.4 % （4-6.3 %）] and 0.5 × 10^4 to 4.7 × 10^5/m3 for suburbs [BioPM% = 10 % （4-8.7 %）]. Distinctive bioaerosol size distribution patterns were observed for different climate zones, e.g., some had fluorescence peaks at 3 μm, while the majority had peaks at 1 μm. Ambient bacterial aerosol community structures were also found different for different geophysical locations. Results suggest that there was a poor overall relationship between PM and BioPM across 40 monitoring locations （R2= 0.081, two-tailed P value = 0.07435）. Generally, city centers had higher PM concentrations than suburbs, but not BioPM and BioPM%. Indoor bioaerosol levels were found at least tenfold higher than those corresponding outdoors. Bacillus was observed to dominate the bacterial aerosol community in the air sample.There is an increasing interest in understanding ambient bioaerosols due to their roles both in health and in climate. Here, we deployed an Ultraviolet Aerodynamic Particle Sizer to monitor viable(fluorescent) bioaerosol concentration levels at city centers(highly polluted) and their corresponding suburbs(near pristine)(total 40 locations) in 11 provinces featuring different climate zones in China between July 16 and 28, 2013. The concentration levels of viable bioaerosol particles(Bio PM) of [0.5 lm were measured, and corresponding percentages of Bio PM%(biological fraction of total PM) and Bio PM2.5%(biological fraction of PM2.5) in particulate matter(PM)and Bio PM, respectively, were determined. For some key cities, indoor viable bioaerosol levels were also obtained.In addition, bacterial structures of the air samples collected across these monitoring locations were studied using pyrosequencing. Bio PM concentration levels ranged from2.1 9 104 to 2.4 9 105/m3 for city centers [Bio PM% =6.4 %(±6.3 %)] and 0.5 9 104 to 4.7 9 105/m3 for suburbs [Bio PM% = 10 %(±8.7 %)]. Distinctive bioaerosol size distribution patterns were observed for different climate zones, e.g., some had fluorescence peaks at 3 lm, while the majority had peaks at 1 lm. Ambient bacterial aerosol community structures were also found different for different geophysical locations. Results suggest that there was a poor overall relationship between PM and Bio PM across 40 monitoring locations(R2= 0.081, two-tailed P value =0.07435). Generally, city centers had higher PM concentrations than suburbs, but not Bio PM and Bio PM%. Indoor bioaerosol levels were found at least tenfold higher than those corresponding outdoors. Bacillus was observed to dominate the bacterial aerosol community in the air sample.

关 键 词：Viable bioaerosol BACTERIA Airpollution - Climate China 

分 类 号：X513[环境科学与工程—环境工程]