A novel and effective hyperthermia method for Schistosomiasis japonica prevention and treatment  

作　　者：Huixia Yang Tao Tang Pei He Youren Zhang Le Xie Guangqi Liu Li Li Weichao Kong Jingdingsha Li Ruiting Liu Yukun Li Junjian Hu Xiaowen Wang Liya Li Guanghong Xiang Geoffrey Gobert Yuesheng Li Jintian Tang 

机构地区：[1]The Yuquan Hospital, Tsinghua University [2]Laboratory of Innovative Medical Technology, Department of Engineering Physics, Tsinghua University [3]The WHO Collaborating Centre for Research and Control of Schistosomiasis on Lake Region, Hunan Institute of Parasitic Diseases [4]Department of Pharmaceutics, Beijing University of Chinese Medicine [5]Graduate School, Hunan University of Chinese Medicine [6]School of Measuring and Optical Engineering, Nanchang Hangkong University [7]Department of Oncology Xiangya Hospital, Central South University [8]Department of Medical Oncology, China-Japan Friendship Hospital [9]Queensland Institute of Medical Research [10]Clinic for Vascular and Endovascular Surgery, University Clinic of Muenster

出　　处：《Science Bulletin》2015年第16期1461-1464,共4页科学通报（英文版）

基　　金：supported by the Bill & Melinda Gates Foundation(Grant No.1024516)

摘　　要：The emerging resistance to schistosoma has been paid much attention and is in urgent need for a novel strategy to control the prevalent parasitic zoonosis. In this study, the efficiency of hyperthermia therapy was investigated by the skin hyperthermia device. The survival rate of cercariae decreased from 68.15 % （37 ℃, 5 min） to 0 （49℃, 10 min） with the thermal dosages increased, which proved the preventing effect of hyperthermia therapy （P 〈 0.05）. Therapeutic effects were assessed in Schistosoma japonicum-infected BALB/c mice. When the cercarial contact region of the skin was treated at 45-49℃ for 5 min within 8 h of infection, worm reduction rate （WRR） reached 74 %-83 % （P 〈 0.01）. The sensitivity of adult schistosoma to heat was also investigated using microwave intraperitoneal hyperthermia （thermal dosages 42-43℃, 20 min）. The WRR, hepatic shift rates and egg reduction rates were 23.7 %, 40 % and 30 %, respectively, comparing with 80.2 %, 59.6 % and 53.9 % of praziquantel （PZQ）-treated group. Encouraging results have been obtained that hyperthermia can effectively kill schistosomula, especially with the appearance of cercarial dermatitis, while PZQ lacks efficacy against the cercariae. Thus, hyperthermia therapy would show significant benefit in preventing and treatment of schistosoma, especially in the early stage.The emerging resistance to schistosoma has been paid much attention and is in urgent need for a novel strategy to control the prevalent parasitic zoonosis. In this study, the efficiency of hyperthermia therapy was investigated by the skin hyperthermia device. The survival rate of cercariae decreased from 68.15 %(37 °C, 5 min) to 0(49 °C, 10 min) with the thermal dosages increased, which proved the preventing effect of hyperthermia therapy(P \ 0.05). Therapeutic effects were assessed in Schistosoma japonicum-infected BALB/c mice. When the cercarial contact region of the skin was treated at 45–49 °C for5 min within 8 h of infection, worm reduction rate(WRR)reached 74 %–83 %(P \ 0.01). The sensitivity of adultschistosoma to heat was also investigated using microwave intraperitoneal hyperthermia(thermal dosages 42–43 °C,20 min). The WRR, hepatic shift rates and egg reduction rates were 23.7 %, 40 % and 30 %, respectively, comparing with 80.2 %, 59.6 % and 53.9 % of praziquantel(PZQ)-treated group. Encouraging results have been obtained that hyperthermia can effectively kill schistosomula, especially with the appearance of cercarial dermatitis, while PZQ lacks efficacy against the cercariae. Thus,hyperthermia therapy would show significant benefit in preventing and treatment of schistosoma, especially in the early stage.

关 键 词：HYPERTHERMIA Schistosomiasis japonica Praziquantel （PZQ） CERCARIAE 

分 类 号：R532.21[医药卫生—内科学]