FXR在高脂血症大鼠肝组织中的表达状况  

FXR expression in liver tissue of hyperlipidemia rats

作　　者：张孟瑜[1] 王洁萍[2] 夏先明[1] 

机构地区：[1]泸州医学院附属医院肝胆外科,四川省泸州市646000 [2]泸州医学院附属医院康复科,四川省泸州市646000

出　　处：《世界华人消化杂志》2015年第23期3755-3760,共6页World Chinese Journal of Digestology

摘　　要：目的:本研究通过建立高脂血症大鼠模型,以法尼基衍生物X受体(farnesyl X receptor,FXR)为研究对象,初步探讨FXR在高脂血症大鼠中的表达状况,为防治高脂血症提供新的依据和分子理论基础.方法:取♂Wistar大鼠70只,随机均分为2组,对照组予以普通饮食、实验组予以高脂饮食.喂食90 d,建立高脂血症模型后,取对照组和实验组肝脏组织:(1)应用逆转录-聚合酶链反应(RT-polymerase chain reaction,RTP C R)技术检测两组模型肝脏组织的F X R基因表达的强度;(2)石蜡切片后用免疫组织化学SP(streptavidin-perosidase)法检测两组模型肝脏组织的FXR蛋白表达的强度.结果:实验组胆固醇及甘油三酯含量明显高于对照组.(1)电泳结果显示:实验中肝脏组织扩增产物都出现了内参β-actin基因的425bp扩增带和FXR基因的283 bp扩增带,实验组FXR基因扩增带亮度强于对照组;(2)免疫组织化学SP法结果显示,实验组FXR表达阳性率为79%,对照组FXR表达阳性率为14.3%,差异具有统计学意义(P<0.05).结论:实验组大鼠的FXR基因的表达较对照组明显增加,提示我们可发展作用于FXR的药物,为高脂血症及其相关疾病找到新的治疗方法.AIM： To establish a rat model of hyperlipidemia, detect the expression of farnesyl X receptor（FXR） in the liver tissue of model rats, and explore the role of FXR in bile acid and cholesterol metabolism in hyperlipidemia rats. METHODS： Sixty Wistar rats were randomly divided into two groups, a control group that was fed an ordinary diet, and an experimental group that was fed a high fat diet. Regular detection of cholesterol and bile acid contents was conducted to assess whether the hyperlipidemia model was successfully established. After successful induction of hyperlipidemia, liver tissue samples were taken to detect the m RNA expression of FXR by reverse transcription-polymerase chain reaction（RT-PCR） and protein expression by immunohistochemistry. RESULTS： In the experiment group, the contents of cholesterol and bile acid were significantly higher than those in the control group. RT-PCR analysis showed that the m RNA expression of FXR in the liver tissue was significantly higher in the experimental group than in the control group. Immunohistochemistry showed that in the experimental group, the positive expression rate of FXR was 79%, significantly higher than 14.3% in the control group（χ2 = 10.862, P〈0.05）. CONCLUSION： FXR expression increases significantly in rats with hyperlipidemia, which suggests that FXR may be used as a target for treatment of hyperlipidemia and related diseases.

关 键 词：FXR基因 胆汁酸 高脂血症 

分 类 号：R589.2[医药卫生—内分泌]