DCPTA对盐胁迫下玉米苗期根系生长、渗透调节及膜透性的影响  

Effects of DCPTA on root growth,osmotic adjustment and cell membrane permeability of maize seedlings under salt stress

作　　者：张翯[1,2] 顾万荣[1,2] 王泳超[1] 李丽杰[1] 曾繁星[1] 杨振芳[1] 杨德光[1,2] 魏湜[1,2] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]农业部东北地区作物栽培科学观测实验站,哈尔滨150030

出　　处：《生态学杂志》2015年第9期2474-2481,共8页Chinese Journal of Ecology

基　　金：国家自然科学基金青年科学基金项目(31201164);十二五国家科技支撑计划粮食丰产科技工程项目(2011BAD16B11);中国博士后基金面上项目(2012M511434)资助

摘　　要：黑龙江省西部地区盐碱土面积约有66.7×104hm2,制约着黑龙江省粮食生产水平的进一步提高。叔胺类活性物质2-(3,4-二氯苯氧基)-乙基-二乙胺(DCPTA)具有提高作物抗逆性作用,为探讨DCPTA提高玉米耐盐性机制,采用营养液水培试验,在前期试验基础之上,选取"先玉335"(耐盐性强)和"丰禾1号"(耐盐性弱)2个品种,研究DCPTA(15mg·L-1)对Na Cl胁迫下(150 mmol·L-1)玉米幼苗根系生长、渗透调节及质膜透性的影响。结果表明:DCPTA能增加盐胁迫下幼苗的根长、根表面积、根体积、根鲜重和根干重,缓解盐胁迫对根系生长的抑制;DCPTA维持了根系水分平衡,提高了盐胁迫下幼苗根系相对含水量,"先玉335"和"丰禾1号"分别提高3.6%和6.4%;DCPTA通过提高根系可溶性糖含量和可溶性蛋白含量,降低幼苗根系脯氨酸含量,增强了盐胁迫下玉米幼苗根系的渗透调节能力,并通过降低根系丙二醛(MDA)含量和相对电导率,保护了根系质膜结构和功能;与盐胁迫相比,"先玉335"丙二醛含量和相对电导率分别降低21.6%和24.2%,"丰禾1号"分别降低28.1%和30.4%;DCPTA缓解盐胁迫对根系伤害的效果表现为"丰禾1号">"先玉335"。The saline-alkali land area is about 667000 hm2 in the western Heilongjiang Province,and the saline-alkali soil restricts the further enhancement of food production level in the province. The diethylaminoethyl-3,4-dichlorophrylether（ DCPTA） is one of tertiary amines active substances,playing an important role in improving crop resistance. The objective of this study was to investigate the mechanism of maize salt tolerance enhanced by DCPTA. A hydroponic experiment was performed with ‘Xianyu 335＇（ salt-tolerant） and ‘Fenghe No. 1＇（ salt-sensitive）to investigate the effects of DCPTA（ 15 mg·L-1） on root growth,osmotic adjustment and cell membrane permeability of maize seedlings under salt stress（ 150 mmol · L-1）. The results showed that exogenous DCPTA application led to a marked increase in root length,root surface,root volume,root fresh weight and root dry weight of seedlings,alleviating growth inhibition under salt stress. In comparison with salt stress,DCPTA could maintain the water balance of roots and improved the relative water contents of ‘Xianyu 335 ＇and ‘Fenghe No. 1 ＇seedlings by3.6% and 6.4%,respectively. DCPTA enhanced the osmotic adjustment ability of maize seedling roots by increasing soluble sugar and soluble protein and decreasing proline content. The exogenous DCPTA may play an important role in stabilizing root plasma membrane structure and function. The malondialdehyde（ MDA） content and relative conductivity of ‘Xianyu 335 ＇ and‘Fenghe No. 1＇under DCPTA pretreatment were decreased by 21.6% and 24.2%,and 28.1%and 30. 4%,respectively. The mitigating effects of DCPTA on root damage by salt stress of‘Fenghe No. 1＇were greater than those of‘Xianyu 335＇.

关 键 词：DCPTA 玉米 耐盐性 根系 渗透调节 

分 类 号：S513[农业科学—作物学]