铜胁迫对拟南芥幼苗生长和基因组DNA甲基化的影响  

Effects of copper stress on the growth and genomic DNA methylation of Arabidopsis thaliana seedlings

作　　者：陈瑞娟[1,2] 何蕾[3] 孙梨宗[1,2] 王鹤潼[1] 宋婕[3] 刘宛[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,污染生态与环境工程重点实验室,沈阳110016 [2]中国科学院大学,北京100049 [3]辽宁大学环境学院,沈阳110016

出　　处：《生态学杂志》2015年第9期2650-2657,共8页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(21347007、21077113、40930739、20977095);辽宁省自然科学基金项目(201202224);国家科技重大专项(2012ZX7505-001)资助

摘　　要：通过Murashige and Skoog（M＆S）培养实验,利用甲基化敏感扩增多态性（methylation-sensitive amplified polymorphism,MSAP）技术研究Cu2＋胁迫对拟南芥幼苗基因组DNA甲基化水平与甲基化模式的变化,同时比较其与幼苗鲜重、根系生长对Cu2＋胁迫的敏感性。结果表明：0、0.25、1.0 mg獉L-1Cu2＋处理15 d后,幼苗根长及鲜重变化差异不显著,而幼苗基因组MSAP率随Cu2＋浓度的增加呈先增高后降低的趋势,分别为15.93%、16.28%和15.83%;高浓度Cu2＋胁迫下（3.0 mg獉L-1）,根长显著变短,鲜重显著降低,MSAP率为14.26%;Cu2＋胁迫（0.25~3.0 mg獉L-1）下,拟南芥幼苗基因组超甲基化（M型）位点及去甲基化（D型）位点数均呈显著增加趋势,Msp I酶较Hpa II酶对胁迫反应更敏感。因此,拟南芥幼苗MSAP变化对低浓度Cu2＋胁迫响应敏感,可作为Cu污染的早期诊断和生态风险评价。In this study,the changes of DNA methylation levels and patterns in Arabidopsis thaliana seedlings under 0- 3.0 mg·L-1Cu2＋stress were studied using methylation-sensitive amplified polymorphism（ MSAP） technique,and the sensitivity of the fresh weight and root growth of seedlings to Cu stress were compared. The results showed that：（ 1） Under Cu stress（ 0.25,1.0mg·L-1） for 15 days,no significant changes were observed in root growth and fresh weight of A.thaliana seedlings compared with the control group,but the genomic MSAP rate first increased and then decreased with the increase of Cu2＋concentration. The MSAP rates were 15. 93%,16.28% and 15.83% at 0,0.25,1.0 mg·L-1Cu2＋,respectively;（ 2） At the high concentration of Cu2＋（ 3.0 mg·L-1）,the root length was shortened,the fresh weight was significantly decreased and the MSAP rate was reduced to 14.26%;（ 3） Under Cu stress（ 0.25-3.0 mg獉L-1）,the number of methylation（ M） type and demethylation（ D） type sites increased significantly,and Msp I was more sensitive to stress response compared to Hpa II. Therefore,the MSAP of A.thaliana was sensitive to low concentration Cu2＋stress,which could be used for early diagnosis and ecological risk assessment of Cu pollution.

关 键 词：MSAP技术 DNA甲基化 拟南芥 CU胁迫 

分 类 号：Q943.2[生物学—植物学]