针刺对焦虑大鼠血浆及胸腺T细胞亚群的影响  

Effects of Acupuncture Intervention on Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats

作　　者：黄文琴[1] 周奇志[1] 刘旭光[1] 魏大能[1] 何薇[1] 张潇丹[1] 彭晓华[1] 

机构地区：[1]成都中医药大学针灸推拿学院,成都610075

出　　处：《针刺研究》2015年第4期265-269,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81173322)

摘　　要：目的:通过观察针刺对焦虑模型大鼠血浆CD 4+、CD 8+T淋巴细胞含量及胸腺细胞CD 4+、CD 8+表达水平的影响,探讨针刺治疗焦虑障碍的部分免疫调节机制。方法:健康SD大鼠34只,随机分为空白组(10只)、模型组(12只)、电针组(12只)。运用慢性不可预知应激刺激方法复制焦虑模型。电针"内关""神门"穴,隔日1次,每次15min,共治疗15d。运用高架十字迷宫测试大鼠焦虑行为,酶联免疫法检测血浆CD 4+、CD 8+的含量,免疫组化法观察成熟胸腺细胞CD 4+、CD 8+的表达。结果:与空白组相比,模型组大鼠在开臂的次数(OE)占进入两臂区停留总次数的百分比(OE%)明显下降(P<0.05);与模型组相比,针刺组OE%值明显提高(P<0.05)。与空白组相比,模型组血浆CD 4+浓度明显下降(P<0.05),CD 8+浓度明显升高(P<0.05),CD 4+/CD 8+比值有降低趋势(P=0.054);针刺可明显提高模型大鼠血浆CD 4+浓度和CD 4+/CD 8+比值(P<0.05,P<0.01),明显降低模型大鼠血浆CD 8+浓度(P<0.05)。与空白组相比,模型组大鼠胸腺髓质CD 4+、CD 8+表达均显著降低(P<0.01);针刺可显著提高模型大鼠胸腺髓质CD 4+、CD 8+的表达(P<0.01)。结论:针刺在发挥抗焦虑效应的同时,还可能通过影响CD 4+、CD 8+的成熟、归巢与再循环,发挥其调节焦虑大鼠T细胞的作用。这可能是针刺调节焦虑障碍免疫功能的重要途径之一。Objective To observe the effect of electroacupuncture（EA）intervention on the levels of CD 4＋and CD 8＋lymphocytes in the plasma and thymus in anxiety disorder rats,so as to explore its mechanism underlying favorable regulation of immune function.Methods Thirty-four SD rats were randomly divided into control（n=10）,model（n=12）and EA（n=12）groups.The anxiety model was established by using chronic unpredictable emotional stress stimulation（fasting,water-deprivation,shaking,tail-clamping,forced warm-and cool-water swimming,electrical shock stimulation,etc.）for 15 days.EA（15Hz/25Hz）was applied to＂Neiguan＂（PC 6）and＂Shenmen＂（HT 7）for 15 min,once every other day for 15 days.The anxiety-like behavior was measured by elevated plus maze test.Anxiety reduction in the plus-maze is indicated by an increase in the proportion of time spent in the open arms（time in open arms/total time in open or closed arms）,and an increase in the proportion of entries into the open arms（entries into open arms/total entries into open or closed arms）.While the contents and expression of CD 4＋and CD 8＋in the plasma and thymus tissues were measured by ELISA and immunohistochemistry,respectively.Results The values of proportions of open-arm entries（OE%）and the content of plasma CD 4＋lymphocytes were obviously lower in the model group than in the control group（P〈0.05）,and were significantly higher in the EA group than in the model group（P〈0.05）.Whereas,the content of plasma CD 8＋lymphocytes was obviously higher in the model group than in the control group（P〈0.05）and markedly down-regulated in the EA group after the treatment（P〈0.05）.The ratio of CD 4＋/CD 8＋was decreased in the model group in comparison with the control group（P=0.054）,and was significantly up-regulated in the EA group after EA stimulation（P〈0.01）.Compared with the control group,the expression levels（optical density,OD values）of CD 4＋and CD 8＋lymphocytes in the thymus tissue were

关 键 词：电针 焦虑 胸腺 T细胞 

分 类 号：R245.97[医药卫生—针灸推拿学]