Effects of electroacupuncture at Chize(LU 5) versus Shangjuxu(ST 37) in rats with ulcerative colitis  

电针尺泽及电针上巨虚对溃疡性结肠炎大鼠的影响比较(英文)

作　　者：罗钦 李志同 杨伟宁 李胜杰 解秸萍 

机构地区：[1]Department of Acupuncture and Massage Beijing University of Chinese Medicine [2]Department of Acupuncture and Massage Changping Hospital of Traditional Chinese Medicine

出　　处：《Journal of Acupuncture and Tuina Science》2015年第4期207-211,共5页针灸推拿医学（英文版）

基　　金：supported by the Independent Subject Project of Beijing University of Chinese Medicine for Postgraduate~~

摘　　要：Objective： To compare the effects between electroacupuncture （EA） at Chize （LU 5, the He-Sea point of the Lung Meridian） and Shangjuxu （ST 37, the lower He-Sea point of the large intestine） in rats with ulcerative colitis （UC） on the variations of mesenteric microcirculation and vasoactive intestinal peptide （VIP） in the colon, lung, and hypothalamus. The relative specificity of acupoints was also explored. Methods： A total of 28 male Wistar rats were randomized into a normal group, a model group, a Chize （LU 5） group and a Shangjuxu （ST 37） group, 7 rats in each group. The UC model was established by enema with acetic acid. Since the third day after modeling, rats in the Chize （LU 5） group and Shangjuxu （ST 37） group respectively received EA at Chize （LU 5） and Shangjuxu （ST 37）, 1S min each time for successive 7 d. The variations of mesenteric microvascular calibers and blood flow status were observed by a microcirculation microscopic tester; VIP in the colon, lung and hypothalamus was measured by radioimmunoassay. Results： Compared with the normal group, the mesenteric microvascular calibers were significantly expanded in the model group （P〈0.05）; there was no significant difference between the model group and Chize （LU 5） group （P〉0.05）; compared with the model group and Chize （LU 5） group, the calibers were obviously shrunk in Shangjuxu （ST 37） group （P〈0.05）. The four groups showed no significant inter-group differences in comparing blood flow status （P〉0.05）. The colonic VIP levels in the model group and Chize （LU 5） group were significantly higher than that in the normal group （P〈0.01, P〈0.05）; the VIP level in Shangjuxu （ST 37） group was markedly lower than that in the model group （P〈0.01）. There were no significant differences among the four groups in comparing VIP level in lung and hypothalamus （P〉0.05）. Conclusion： The effects of Chize （LU 5） and Shangjuxu （ST 37） were different 目的:比较电针尺泽(肺经合穴)与上巨虚(大肠下合穴)对溃疡性结肠炎(ulcerative colitis,UC)大鼠肠系膜微循环及结肠、肺、下丘脑中血管活性肠肽(vasoactive intestinal peptide,VIP)的不同影响,并探讨腧穴相对特异性规律。方法:将28只Wistar雄性大鼠随机分为正常组、模型组、尺泽组及上巨虚组,每组7只。采用乙酸灌肠法制备UC模型。灌肠后第3天起,尺泽组与上巨虚组每日分别电针尺泽穴、上巨虚穴15 min,连续治疗7天后取材。微循环仪观察肠系膜微血管管径及血液流态变化;放射免疫法测定结肠、肺及下丘脑VIP含量。结果:模型组肠系膜微循环管径较正常组显著扩大(P<0.05);模型组与尺泽组无显著差异(P>0.05);上巨虚组较模型组、尺泽组显著缩小(P<0.05)。各组血液流态无统计学差异(P>0.05)。模型组、尺泽组结肠VIP含量较正常组显著升高(P<0.01,P<0.05);上巨虚组较模型组显著下降(P<0.05)。各组肺、下丘脑VIP含量无显著差异(P>0.05)。结论:尺泽穴和上巨虚穴对UC效应不同,上巨虚穴能降低UC大鼠结肠VIP,改善肠道微循环,尺泽穴效应不明显。

关 键 词：Acupuncture Therapy ELECTROACUPUNCTURE Point  Chize （LU 5） Point  Shangjuxu （ST 37） Colitis  Ulcerative MICROCIRCULATION Vasoactive Intestinal Peptide 

分 类 号：R2-03[医药卫生—中医学]