氢气对七氟醚麻醉诱发新生大鼠海马神经元凋亡的影响  

Effects of hydrogen on apoptosis in hippocampal neurons caused by sevoflurane anesthesia in neonatal rats

作　　者：丁玲[1] 敖吉莹 汤晓红[1] 张麟临 李楠[1] 王志芬[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科天津市麻醉学研究所,300052

出　　处：《中华麻醉学杂志》2015年第6期687-689,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81371245、30972847、81071059、81100984）;天津市科技支撑计划重点项目（12ZCZDSY03000）;天津市卫生局科技基金（09KZ106）

摘　　要：目的 评价氢气对七氟醚麻醉诱发新生大鼠海马神经元凋亡的影响.方法 健康雄性SD大鼠48只,7日龄,体重12～20 g,采用随机数字表法,将其分为3组（n=16）：对照组（C组）、七氟醚麻醉组（S组）和氢气组（H组）.C组吸入30％氧气6h;S组吸入3％七氟醚6h;H组吸入3％七氟醚和2％氢气6h.于出生后7d（吸入氧气、七氟醚或氢气结束后）,每组处死8只大鼠,取海马组织,采用Western blot法测定活化的caspase-3和髓磷脂碱性蛋白的表达水平.于出生后28 d时,取8只大鼠,行Y迷宫实验及Morris水迷宫实验测定认知功能,记录进入各臂总次数、自发交替次数、逃避潜伏期和原平台所在象限滞留时间.结果 与C组比较,S组自发交替百分率降低,逃避潜伏期延长,原平台所在象限滞留时间缩短,海马组织活化的caspase-3表达上调,髓磷脂碱性蛋白表达下调（P＜0.05）;与S组比较,H组自发交替百分率升高,逃避潜伏期缩短,原平台所在象限滞留时间延长,海马组织活化的caspase-3表达下调,髓磷脂碱性蛋白表达上调（P＜0.05）;3组间进入各臂总次数比较差异无统计学意义（P＞0.05）.结论 氢气可抑制七氟醚麻醉诱发新生大鼠海马神经元凋亡.Objective To evaluate the effects of hydrogen on apoptosis in hippocampal neurons caused by sevoflurane anesthesia in neonatal rats.Methods Forty-eight healthy male Sprague-Dawley rats,aged 7 days,weighing 12-20 g,were randomly divided into 3 groups （n=16 each） using a random number table：control group （group C）;sevoflurane anesthesia group （group S）;hydrogen group （group H）.In C and S groups,the rats inhaled 30% oxygen and 3% sevoflurane for 6 h,respectively.In group H,3% sevoflurane and 2% hydrogen were inhaled for 6 h.Eight rats in each group were randomly selected and sacrificed at 7 days after birth （after the end of oxygen,sevoflurane or hydrogen inhalation）,and the hippocampus was removed for determination of the expression of activated caspase-3 and myelin basic protein by Western blot.At 28 days after birth,8 rats were selected,and Y-maze and Morris water maze tests were performed to evaluate the cognitive function.The total number of entries into each arm,the number of spontaneous alternation,escape latency and time of staying at the platform quadrant were recorded.Results Compared with group C,the percentage of spontaneous alternation was significantly decreased,the escape latency was prolonged,and the time of staying at the platform quadrant was shortened,and the expression of activated caspase-3 was significantly up-regulated,and the expression of myelin basic protein was down-regulated in group S.Compared with group S,the percentage of spontaneous alternation was significantly increased,the escape latency was shorten,and the time of staying at the platform quadrant was prolonged,and the expression of activated caspase-3 was significantly downregulated,and the expression of myelin basic protein was up-regulated in group H.There was no significant difference in the number of entries into each arm in Y-maze test between the three groups.Conclusion Hydrogen can inhibit apoptosis in hippocampal neurons caused by sevoflurane anesthesia in neonatal rats.

关 键 词：氢气 麻醉药 吸入 麻醉 婴儿 新生 细胞凋亡 

分 类 号：R614[医药卫生—麻醉学]