新生期反复伤害性刺激对大鼠成年期痛敏感性的影响  

Effects of repetitive noxious stimuli during neonatal period on pain sensitivity during adulthood in rats

作　　者：夏冬晴[1] 陈梦莹[1] 闵翠婷 戴焱焱[1] 周楠[1] 李晓南[1] 

机构地区：[1]南京医科大学附属南京儿童医院儿童保健科,210008

出　　处：《中华麻醉学杂志》2015年第6期718-720,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81202222）

摘　　要：目的 评价新生期反复伤害性刺激对大鼠成年期痛敏感性的影响.方法 清洁级健康雄性新生SD大鼠20只,体重6.2～6.8 g,采用随机数字表法分为对照组（C组）和反复伤害性刺激组（RNS组）,每组10只.RNS组采用28 G型细针,按顺序分别针刺4足背,1次/6 h,自出生开始,持续7d,C组以相同模式行触觉刺激.出生后第10周右足皮下注射完全弗氏佐剂诱发大鼠炎性痛,于注射前、注射后24 h和7 d（T0-2）时测定机械痛阈和热痛阈,T2时行酒精偏好实验.结果 与左足比较,2组T1,2时右足机械痛阈降低（P＜0.05）;与C组比较,RNS组T0-2时双足机械痛阈及T1时右足热痛阈降低,酒精摄入百分比升高（P＜0.05）.结论 新生期反复伤害性刺激可导致大鼠成年期痛敏感性显著升高.Objective To evaluate the effects of repetitive noxious stimuli during the neonatal period on the pain sensitivity during adulthood in rats.Methods Twenty pathogen-free male neonatal SpragueDawley rats,weighing 6.2-6.8 g,were randomly divided into 2 groups （n=10 each） using a random number table：control group （group C） and repetitive noxious stimuli group （group RNS）.In RNS group,neonatal rats sequentially received needle pricks into the dorsal surface of 4 paws at 6 h intervals with a 28 G needle per day during the first postnatal week.In group C,the animals received non-painful tactile stimuli with cotton tip rub.At the age of 10 weeks,complete Freund＇ s adjuvant was injected into the plantar surface of the right hind paw to induce inflammatory pain.Before injection,and at 24 h and 7 days after injection （T0-2）,the mechanical and thermal thresholds were measured,and alcohol preference test was performed for assessment of the behavior at T2.Results The mechanical threshold of the right paws was significantly lower at T1.2 than that of the left paws in the two groups.Compared to group C,the mechanical threshold of the left and right paws at T0-2 and thermal threshold of the right paws at T1 were significantly decreased,and alcohol intake was increased in group RNS.Conclusion Repetitive noxious stimuli during the neonatal period can lead to increase in the pain sensitivity during adulthood in rats.

关 键 词：婴儿 新生 痛觉过敏 疼痛 成年人 

分 类 号：R722[医药卫生—儿科]