CCR3在哮喘小鼠气道组织Muc5ac表达中的作用  

Effects of CCR3(Chemokine receptor-3) on Muc5ac in airway of asthmatic mice

作　　者：王涛[1] 张建勇[1] 王秀丹[1] 郑飞彦 杨红霞[1] 

机构地区：[1]遵义医学院附属医院呼吸二科,遵义563000

出　　处：《中国免疫学杂志》2015年第8期1019-1023,共5页Chinese Journal of Immunology

基　　金：贵州省科学技术基金项目(黔科合J字[2007]2018号);贵州省科技厅基金项目(黔科合SY字[2008]3065)

摘　　要：目的:探讨CCR3在哮喘小鼠气道黏蛋白5ac(Muc5ac)表达中的作用。方法:将50只清洁级BALB/c小鼠随机分为正常对照组、哮喘组、地塞米松干预组、SB328437干预组及溶剂对照组二甲基亚砜组,各10只。测定BALF中细胞总数、分类,ELISA检测IL-4、TNF-α,肺组织HE染色,免疫组化和RT-PCR检测Muc5ac、CCR3的表达。结果:在BALF细胞总数、嗜酸性粒细胞、单核细胞、淋巴细胞、IL-4,TNF-α水平、AB-PAS阳染面积、黏蛋白Muc5ac、CCR3蛋白IOD及Muc5ac mRNA、CCR3 mRNA表达方面,哮喘组与地塞米松干预组、SB328437干预组,具有统计学差异(P<0.01或P<0.05)。结论:SB328437可抑制肺组织CCR3表达,进而抑制Muc5ac的表达和分泌,从而控制气道炎症。Objective： To investigate the effects of CCR3 on Muc5 ac in the airway of asthmatic mice. Methods： Fifty clean BALB / c mice were randomly divided into control group,asthmatic group,dexamethasone treatment group,SB328437 treatment group,vehicle-control group. Total cells and differential inflammatory cells were counted in BALF,the levels of IL-4 and TNF-α were determined by ELISA,lung tissue HE staining the expressions of Mucin5ac（ Muc5ac） and CCR3 in lung tissue were detected by immuneohistochemical staining and RT-PCR. Results： The total cells,eosinophil,monocytes and lymphocyte cells in BALF,the levels of IL-4,TNF-α in BALF,the goblet cell of airway wall,the expression of Muc5 ac and CCR3 positive staining IOD in the airway and the expression of Muc5 ac and CCR3 mRNA lung tissue obviously decreased in SB328437 treatment group and DEX treatment group which compared with asthmatic mice model group（ P〈0. 05）. Conclusion： SB328437 can inhibit the expression of CCR3 in pulmonary tissue,furtherly inhibit the expression and secretion of Muc5 ac and control the airway inflammation.

关 键 词：哮喘 小鼠 黏蛋白5ac(Muc5ac) 趋化因子受体3拮抗剂 

分 类 号：R562.2[医药卫生—呼吸系统]