戊二醛固定的异种血管的先天性免疫机制研究  

Innate immunology research of glutaraldehyde-treated xenogenic blood vessels

作　　者：张颖[1] 龚达[1,2] 辛毅[1] 崔巍[1] 刘洁琳[1] 万居易[1] 刘飒[1] 李殿坤[1] 杜兰平[1] 辛志飞 许秀芳[1] 李温斌[1] 

机构地区：[1]首都医科大学附属北京安贞医院,北京市心肺血管疾病研究所,北京100029 [2]北京大学国际医院,北京102206

出　　处：《中国免疫学杂志》2015年第8期1085-1088,1093,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.81270217和No.81070077);北京市高级卫生人才学术骨干(No.2009-3-44和No.2013-3-012);省部共建重点教育部心血管重塑相关疾病重点实验室项目(No.110267)

摘　　要：目的:系统进行戊二醛固定的异种血管的免疫学研究及其动物在体排斥反应强度。方法:实验分两组:新鲜未处理组和戊二醛固定组。每一组分术后第4天、12天、30天和60天四个时间点。分别通过对20例新鲜猪肺动脉、20例戊二醛固定的异种血管进行大鼠皮下包埋,在术后第4天、12天、30天和60天进行观察,利用病理学和免疫组织化学等技术,全面系统比较了两组病理学、巨噬细胞CD68、补体C3、先天性抗体Ig G形态性变化及其IOD值变化。结果:HE染色结果显示新鲜组在术后第12天、30天染色较强,戊二醛组以上指标术后第12天、30天染色比新鲜组较弱。CD68、C3、Ig G值变化结果相似;免疫组织化学累积光密度(Integral optical density,IOD)定量检测结果显示:IOD值术后第4天开始升高,到第12天、30天达到峰值,第60天维持或下降。结论:先天性免疫在探索异种免疫排斥机理有重要作用;戊二醛固定的异种血管免疫原性下降,但是仍然具有免疫原性,需要探索更好的方法来削弱排斥反应。Objective： To conduct a systematic study of the immunologic response of rats to transplanted glutaraldehyde（ GA）-treated porcine blood vessels in vivo. Methods： The experiment was divided into two groups： fresh group and glutaraldehyde-treated group. Twenty cases of fresh and glutaraldehyde-treated porcine pulmonary arteries were subcutaneously embedded in rats. We compared the changes using HE staining and immunohistochemistry. Results： HE staining showed that there were stronger expression on day 12 and day 30 in the fresh group than that in the glutaraldehyde group. There were similar results in morphology in CD68,C3,Ig G. The results of integral optical density（ IOD） in immunohistochemistry showed that IOD started rising from day 4 and got the peak on day 12 or day 30 and or fell on day 60. Conclusion： Innate immunity played an important role in the research on xenogenic immunological rejection mechanism. The immunogenicity of glutaraldehyde-treated xenogenic blood vessels is lower than that in fresh blood vessels. However there is still immunogenicity in glutaraldehyde-treated xenogenic blood vessels. We will explore better ways to obviously weaken the rejection.

关 键 词：大鼠皮下包埋 异种移植排斥反应 免疫组织化学 先天性免疫 

分 类 号：R392.4[医药卫生—免疫学]