外周血滤泡辅助性T细胞与B细胞在酒精性股骨头坏死患者中的作用  

Function and correlation of follicular helper T cells and B cells in alcohol abuse non-traumatic osteonecrosis of femoral head

作　　者：丁福鹏[1] 戚长林 高辉[1] 刘易军[1] 王井[1] 单玉兴[1] 

机构地区：[1]吉林大学第一医院分院骨科,长春130021

出　　处：《中国免疫学杂志》2015年第8期1109-1112,1119,共5页Chinese Journal of Immunology

摘　　要：目的:探讨酒精相关性股骨头坏死患者外周血滤泡辅助性T细胞、B细胞、白细胞介素(IL)-21的表达水平与疾病临床相关指标的相关性及其在酒精性股骨头坏死发病机制中的作用。方法:流式细胞仪测定酒精性股骨头坏死患者和健康对照组外周血中Tfh和B细胞的水平;ELISA法检测患者和健康对照血清中IL-21的表达水平。结果:酒精性股骨头坏死患者外周血CD19+B细胞、CD86+CD19+B细胞、CD95+CD19+B细胞Tfh细胞、PD-1+Tfh细胞和IL-21+Tfh细胞百分率高于对照组;外周血Tfh细胞百分率与CD19+B细胞百分率呈正相关,外周血IL-21+Tfh细胞百分率与CD86+CD19+B细胞百分率呈正相关,CD27+CD19+B细胞较对照组明显减少,CD86+CD19+B细胞百分率与股骨头坏死评分呈正相关。结论:酒精性股骨头坏死患者外周血Tfh细胞及B细胞水平显著增高,CD86+B细胞与疾病进展具有相关性,且Tfh细胞与B细胞的变化在疾病中具有显著的相关性,提示Tfh-B细胞相互作用在酒精性股骨头坏死的发病过程中发挥着重要作用。Objective： To examine the roles of follicular helper T（ Tfh） cells,serum IL-21 and B cells in the pathogenesis of alcohol abuse non-traumatic osteonecrosis of the femoral head（ NONFH）. Flow cytometry was used to measure the frequencies of peripheral blood inducible Tfh cells and B cells in alcohol abuse NONFH patients and healthy controls. The disease progression and the extent of femoral head collapse,the serum IL-21 were quantified. Methods： A significantly higher percentages of CD19^＋B cells（ t = 3. 765,P =0. 0005）,CD86^＋CD19^＋B cells（ t = 5. 506,P〈0. 0001）,and CD95＋CD19^＋B cells（ t = 4. 152,P = 0. 0002） in patients than those in controls was found. The percentages of CD86^＋CD19^＋B cells were positively associated with the index of femoral head collapse in alcohol abuse NONFH（ P〈0. 0001,r = 0. 536）. Results： The frequencies of Tfh cells（ t = 7. 611,P〈0. 0001）,and IL-21^＋Tfh cells（ t =5. 281,P〈0. 0001） were higher than those in controls; The frequencies of Tfh cells were positively associated with the percentages of CD19^＋B cells（ P = 0. 0002,r = 0. 455）,IL-21^＋Tfh cells were positively associated with the percentages of CD86^＋CD19^＋B cells（ P =0. 0002,r = 0. 447）. Conclusion： Tfh cells and B cells may participate in the pathogenesis of alcohol abuse NONFH,and increased CD86^＋CD19^＋B cells may be associated with the extent of femoral head collapse,the interaction of Tfh cells and B cells may have an important role in pathogenesis of alcohol abuse NONFH.

关 键 词：酒精性股骨头坏死 滤泡辅助性T细胞 B细胞 白细胞介素21 

分 类 号：R392.3[医药卫生—免疫学]