D-柠檬烯对酒精性肝损伤大鼠铁过载调节作用  

Effect of D-limonene on iron overload in rats with alcoholic liver injury

作　　者：孙婷婷[1] 梁惠[1] 刘颖[2] 王文成[1] 戈娜[3] 刘靖靖[1] 

机构地区：[1]青岛大学公共卫生学院,山东青岛266021 [2]青岛大学基础学院 [3]内蒙古科技大学包头医学院

出　　处：《中国公共卫生》2015年第9期1176-1179,共4页Chinese Journal of Public Health

基　　金：国家"十二五"科技支撑计划项目(2012BAD33B01);国家自然科学基金(31171671)

摘　　要：目的建立酒精性肝损伤大鼠模型,探讨右旋柠檬烯(D-柠檬烯)对酒精性肝损伤大鼠铁过载的调节作用。方法 70只Wistar雄性大鼠,适应性喂养7 d,随机分为7组,每组10只,即对照组、模型组(酒精性肝损伤)、柠檬烯低、中、高剂量组(100、200、400 mg/kg)、柠檬烯对照组(400 mg/kg)、甘利欣组,给药6周后处死大鼠;苏木素-伊红(HE)染色观察肝组织形态结构;透射电镜观察肝脏超微结构变化,电感耦合等离子体质谱(ICP-MS)测大鼠肝脏内铁含量(LIC);酶联免疫吸附法测血浆铁调素(HEPC)、铁蛋白(SF)和血幼素(HJV)水平。结果 HE染色结果显示,模型组大鼠肝小叶结构紊乱,肝组织呈弥漫性大泡性脂肪变性,肝小叶纤维组织增生明显,可见点灶状坏死;与模型组比较,柠檬烯组大鼠肝小叶结构紊乱程度、肝细胞脂肪变性程度和损害程度明显减轻;透射电镜结果显示,与模型组比较,柠檬烯组大鼠肝内质网、线粒体结构均有明显改善,脂滴明显减少;与对照组比较,模型组大鼠LIC、SF水平[分别为(279.14±5.87)μg/m L、(57.76±5.83)ng/m L]升高,HEPC、HJV含量[分别为(61.85±4.85)、(46.21±5.24)ng/m L]降低(P<0.05);与模型组比较,高剂量柠檬烯组大鼠LIC、SF水平[分别为(180.14±5.76)μg/m L、(35.39±3.94)ng/m L]明显降低,HEPC、HJV含量[分别为(81.36±4.8)、(67.29±5.89)ng/m L]明显升高(P<0.05)。结论 D-柠檬烯对慢性酒精暴露大鼠肝脏损害所致铁过载具有一定拮抗作用。Objective To establish a rat model of alcoholic liver injury and to explore the effect of D-limonene on iron overload in rats with chronic alcoholic liver injury. Methods Seventy male wistar rats were fed adaptively for seven days and then randomly divided into seven groups （n = 10 for each group）, including a control group, a alcohol-model group （alcoholic liver damage）, the low, medium and high D-limonene treated groups （100,200,400 mg. kg 1. day- 1 ）, a limonene control group （400 mg/kg）, and a glycyrrhizic acid group. The experiment lasted for six weeks. The histopa- thology of the liver was observed after hematoxylin-eosin （HE） staining. Ultrastructural changes of the livers were observed with transmission electron microscope （TEM）. The liver iron content was measured with inductively coupled plas- ma-mass spectrometry （ ICP-MS ）. In addition, the levels of plasma hepcidin, plasma ferritin and plasma hemojuvelin （HJV） were assessed with enzyme-linked immunosobent assay （ELISA）. Results In the alcohol-model group, the struc- ture disturbance of hepatic lobule,the diffuse fatty degeneration in the liver tissue,and obvious hyperplasia of the hepatic fibrous tissue with visible spotty necrosis were observed. Compared with those of the alcohol-model group, the degree of structure disturbance in hepatic lobule,liver fatty degeneration and damage were all significantly reduced in various doses D-limonene groups. TEM showed that hepatic ultrastructure lesions, including the structure of endoplasmic reticulum, mi- tochondria and lipid droplet, were reduced in different doses D-limonene groups compared with alcohol-model group. The rats in alcohol-model group had a significant increase in liver iron content（279. 14 ± 5.87 μg/mL）and level of plasma ferritin（57. 76 ± 5.83 ng/mL）（P 〈 0. 05） compared with those of the normal group;but both the level of hepcidin （61.85 ± 4. 85 ng/mL）and HJV（46. 21 ± 5.24 ng/mL）were obviously decreased （P 〈 0. 05 ）. Compar

关 键 词：右旋柠檬烯(D-柠檬烯) 酒精性肝损伤 铁过载 肝脏铁浓度(LIC) 铁调素(HEPC) 铁蛋白(SF) 血幼素(HJV) 

分 类 号：R931.71[医药卫生—生药学] R285.5[医药卫生—药学]