丙醇/硫酸铵双水相体系分离纯化茶皂素  

Separation and Purification of Tea Saponin with Propyl Alcohol /Ammonium Sulphate Aqueous Two-Phase System

作　　者：张团结[1] 熊道陵[1] 陈超[1] 许光辉[1] 陈金洲 

机构地区：[1]江西理工大学冶金与化学工程学院,江西赣州341000

出　　处：《精细化工》2015年第9期1009-1013,共5页Fine Chemicals

基　　金：国家自然科学基金资助项目(51364014);江西省科技厅资助项目(2013BBG7003);江西省大学生创新创业训练计划项目(201310407037;201310407057;201410407030);江西省高校专业综合改革试点项目(SZG-14-01-01)~~

摘　　要：以茶饼中提取的茶皂素为粗品原料(茶皂素质量分数为40.2%),采用丙醇/硫酸铵双水相体系对粗品茶皂素进行分离提纯。考察了硫酸铵质量分数、丙醇质量分数、萃取温度、萃取p H对茶皂素产品萃取率和产品含量的影响。结果表明,该工艺分离速度快,产品萃取率和含量较高,在硫酸铵质量分数为20%、丙醇质量分数为23%、温度为40℃、p H为7时,茶皂素萃取率可达到89.32%,茶皂素质量分数可达到78.12%。With the crude saponin（ 40. 2%） extracted from tea cake as raw materials,an aqueous twophase system based on propyl alcohol / ammonium sulfate was used for the separation and purification of saponin. The influences of factors such as the concentration of ammonium sulfate,the concentration of propyl alcohol,temperature and p H were investigated. The results show that the process guaranteed speedy separation with a much higher product extraction ratio and content. The optimum process conditions were as follows： ammonium sulfate concentration 20%,propyl alcohol concentration 23%,temperature 40 ℃ and p H 7. Under the optimal conditions,the extraction ratio and the content reached89. 32% and 78. 12%,respectively.

关 键 词：茶皂素 双水相萃取 萃取率 提纯技术 

分 类 号：TQ423.2[化学工程]