梅毒螺旋体不同接种途径对家兔致病性研究初探  

Pathogenicity of Treponema pallidum inoculated through different routes in rabbits： a preliminary study

作　　者：王琪[1] 尹跃平[1] 龚匡隆[1] 

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》2015年第9期621-624,共4页Chinese Journal of Dermatology

摘　　要：目的探讨不同接种途径建立的梅毒家兔模型的发病及免疫应答情况。方法20只家兔随机分为睾丸接种组及背部皮内接种组，每组10只。睾丸接种组家兔睾丸注射2×10^7梅毒螺旋体Nichols株。背部皮内接种组家兔背部剃毛后选取6个点进行皮内注射，6个点共注射2×10^7梅毒螺旋体Nichols株。观察两组家兔的临床症状及血清学反应情况。结果睾丸接种组接种后第8天，睾丸开始肿大变硬，13—16d肿大最为明显，之后逐渐缩小，至接种后28d所有家兔睾丸回缩，触诊未见肿大变硬；发病2个月内未发现其他部位出现皮损。背部皮内接种组接种后7d，接种部位皮肤出现红肿，15—45d皮损红肿最为明显，且出现溃疡，有3只家兔共12个接种点的皮肤发生溃疡，暗视野检查阳性，触诊呈软骨样硬度。睾丸接种组接种后（14．50±1．08）d时临床症状最为明显，皮内接种组（29．00±10．30）d时临床症状最为明显，两组比较，t=5．02，P〈0．05。两组家兔快速血浆反应素环状卡片试验（RPR）最高滴度分布经秩和检验，差异有统计学意义（P〈0．05），睾丸接种组滴度高于背部皮内接种组。结论梅毒螺旋体接种家兔不同部位，引起家兔不同的临床症状及免疫反应。睾丸接种组RPR阳性时间早，且滴度高。背部皮内接种组的临床症状类似于人类一期梅毒硬下疳表现。Objective To observe clinical manifestations and immune responses in rabbit models of syphilis established by inoculation with Treponema pallidum through different routes. Methods A total of 20 rabbits were randomly and equally divided into two groups： testis-inoculated group injected with Treponema pallidum （Tp） suspensions into the testes, back-inoculated group intracutaneously injected with Tp suspensions at six sites on the shaved back. All the rabbits received a single injection with the total injection amount of Tp （Nichols strains） being 2 x 107 in both groups. Clinical symptoms and immune responses were evaluated in both groups every other day. Statistical analysis was carried out by a two-sample t-test and rank sum test with the SPSS 20.0 software. Results On day 8 after the inoculation, the testes of rabbits in the testis-inoculated group started to swell with induration, and the swelling was most severe during days 13 - 16. Afterwards, the testes gradually diminished, and returned to normal in size without swelling or hardening on day 28. No skin lesions occurred in the other sites in the testis-inoculated group within 2 months after the inoculation. Erythematous swelling occurred at inoculation sites on day 7 after inoculation in the backinoculated group, which was most obvious between days 15 and 45. Moreover, cartilage-like indurated ulcers were observed at 12 inoculation sites in 3 rabbits in the back-inoculated group, and dark-field examination of the ulcers showed the presence of Tp. There was a significant difference in the time required for the. severity of lesions to peak between the testis-inoculated group and back-inoculated group （ 14,50 ± 1.08 days vs. 29.00 ± 10.30 days, t = 5.02, P〈 0.05）. Rank sum test showed significant differences in the distribution of highest RPR titers between the two groups （P 〈 0.05）, and RPR titers were higher in the testis-inoculated group than in the back-inoculated group. Conclusions The injection of Tp suspensions at different sites c

关 键 词：梅毒 苍白密螺旋体 疾病模型 动物 兔 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]