活化抗原CD69、HLA—DR在寻常性银屑病外周血单一核细胞和皮损中的表达  

Expressions of activation antigens CD69 and HLA-DR in peripheral blood mononuclear cells and skin lesions of patients with psoriasis vulgaris

作　　者：陈文娟[1] 顾俊瑛[1] 龚瑜[1] 刘至昱[1] 许辉[1] 熊惠姊 史玉玲[1] 

机构地区：[1]同济大学附属第十人民医院皮肤科,上海200072

出　　处：《中华皮肤科杂志》2015年第9期625-628,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81301356）;上海市自然科学基金（13ZR1432200）;上海市科委科研计划引导项目（134119b0700）

摘　　要：目的探讨活化抗原CD69和HLA—DR在寻常性银屑病患者外周血CD3CF淋巴细胞和皮损中的表达变化。方法流式细胞仪检测20例寻常性银屑病患者和20例健康对照外周血CD3＋T细胞中CD69和HLA—DR的表达，同时采用免疫组化的方法检测15例寻常性银屑病患者和10例健康对照皮损组织中HLA—DR的表达并进行对比。两组统计学分析采用双侧t检验。结果CD69和HLA—DR在寻常性银屑病患者外周血CD3＋T细胞中的表达率分别为（4．70±1．90）％、（8．97±1．79）％，比健康对照组[（1．56±0．95）％、（3．02±1．15）％]明显升高（均P〈0．01）。Pearson相关性分析发现，CD3＋HLA—DR＋细胞百分比与银屑病皮损和严重程度评分指数（PASI）评分呈正相关（r=O．5626，P〈0．05）。与健康对照组比较，寻常性银屑病患者皮损真皮中HLA—DR表达升高，但表皮中HLA—DR表达降低（均P〈0．05），HLA—DR在健康人主要分布在血管周围，而在寻常性银屑病患者则广泛表达于真皮组织。结论寻常性银屑病患者外周血CD3CF淋巴细胞及皮损中的炎症细胞存在异常活化，外周血CD3＋HLA—DR＋细胞与疾病严重程度存在相关性。Objective To investigate changes in expressions of activation antigens CD69 and HLA-DR in CD3＋ T lymphocytes in peripheral blood and skin lesions in patients with psoriasis vulgaris. Methods Peripheral blood samples were obtained from 20 patients with psoriasis vulgaris and 20 healthy controls, and skin specimens from the lesions of 15 out of the 20 patients and 10 healthy controls. Flow cytometry was performed to quantify the expressions of CD69 and HLA-DR in peripheral blood CD3＋ T cells, and an immunohistochemical study to measure the expression of HLA-DR in skin specimens. Statistical analysis was carried out by a two-sample t-test and Pearson correlation analysis with the SPSS 19.0 software. Results Compared with the healthy controls, the patients with psoriasis vulgaris showed increased expression rates of CD69 （4.70% ±1.90% vs. 1.56% ±0.95%, t = 6.629, P 〈 0.01 ） and HLA-DR （8.97% ± 1.79% vs. 3.02% ±1.15%, t = 6.204, P 〈 0.01 ） in peripheral blood. Pearson correlation analysis revealed that the percentage of CD3＋HLA-DR＋ cells in peripheral blood was positively correlated with the psoriasis area and severity index （PASI） score （r = 0.5626, P 〈 0.05 ）. The expression rate of HLA-DR was significantly higher in the dermis （64.87% ± 17.31% vs. 19.80%±5.69%, t = 7.916, P 〈 0.01 ）, but lower in the epidermis （ 11.80% ± 5.55% vs. 27.40% ± 8.61%, t = 5.479, P〈 0.01） in the psoriatic specimens compared with the control specimens. Immunohistochemically, HLA-DR was widely expressed in the dermis of psoriatic lesions, but mainly distributed around blood vessels in the control skin. Conclusions There is an aberrant activation of CD3＋ T cells in peripheral blood and inflammatory ceils in skin lesions in patients with psoriasis vulgaris, and the percentage of CD3 ＋HLA-DR＋ ceils in peripheral blood is correlated with the severity of psoriasis vulagaris.

关 键 词：银屑病 抗原 CD69 HLA—DR抗原 T淋巴细胞 淋巴细胞活化 疾病严重程度指数 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]