2015年我国部分地区2.1d新基因亚型猪瘟病毒的分子流行病学分析  

Molecular epidemiological analysis for new subgenotype 2.1d of classical swine fever virus in parts of China in 2015

作　　者：冯丽苹[1] 张洪亮[1] 刘春晓[1] 冷超粮[2] 陈家锃[1] 李真[1] 彭金美[1] 王倩[1] 白云[1] 张武超[1] 安同庆[1] 蔡雪辉[1] 童光志[3] 田志军[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]南阳师范学院,河南南阳473061 [3]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国预防兽医学报》2015年第9期651-655,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省杰出青年科学基金(JC201314);国家自然科学基金(31001050/C1803)

摘　　要：为了解猪瘟病毒（CSFV）在我国的流行情况，本研究对2015上半年来自4个省疑似猪瘟（csF）病料样品采用RT．PCR方法进行检测，28份样品中14份为CSFV阳性，并对这些阳性样品进行CSFVE2基因遗传进化分析。结果表明，14个病毒株中11个属于2．1d新基因亚型，它们与CSFV代表株Shimen、HCLV和2．1b基因亚型病毒株的核苷酸同源性分别为82．8％～84．1％、81．1％～82．4％和92．6％～97．1％，氨基酸同源性分别为89．8％～91．2％、88．2％～89．8％和94．1％～98．9％，其余3个病毒株属于2．1b亚型。这些2．1d亚型新分离株在E2基因的4个氨基酸（R31、S34、K303和A331）上具有相同的分子特征。本研究结果与我们2014年CSFV流行病学研究结果一致，表明2．1d亚型病毒株已成为我国现阶段CSFV的主要流行株。To investigate the prevalence of classical swine fever virus （CSFV） in China, twenty-eight clinical samples were collected in pigs from suspected CSFV-infected farms in 4 provinces （Shandong, Hebei, Jilin and Heilongjiang） of China in early 2015 which were subjected to detection and identification of subgenotype CSFV. The results showed 14 of the 28 samples were positive for CSFV, and E2 gene sequences of the 14 CSFVs were sequenced, aligned and analyzed, of which the 11/14 CSFVs in 2015 belonged to the 2.1d subgenotype, and 3/14 belonged to the 2.1b subgenotype. In addition, compared with the Shimen, HCLV and the 2. lb subgenotype CSFV strains, the nucleotides similarity of the 11 new 2. ld subgenotype CSFVs were 82.8% to 84.1%, 81.1% to 82.4% and 92.6% to 97.1%, and similarity of the deduced amino acids were 89.8% to 91.2%, 88.2% to 89.8% and 94.1% to 98.9%, respectively. Furthermore, 2. l d subgenotype CSFVs was of the same molecular characteristics, which were R31, S34, K303 and A331 in E2 gene. The study results were consistent with the related study in 2014. Importantly, the results suggested that 2. ld subgenotype of CSFVs had become the major prevalent strains of CSFV in China.

关 键 词：猪瘟病毒 新基因亚型 流行病学 

分 类 号：S852.65[农业科学—基础兽医学]